机构：[1]The State Key Lab of Respiratory Disease, The First Affiliated Hospital,Guangzhou Medical University, 151 Yanjiangxi Road, Guangzhou 510120,China[2]The School of Public Health, The Institute for Chemical Carcinogenesis,Collaborative Innovation Center for Environmental Toxicity, GuangzhouMedical University, Xinzao, Panyu District, Guangzhou 511436, China[3]Department of Genetics, Medical College of Soochow University, 1 ShiziRoad, Suzhou 215123, China[4]School of Public Health, Heping DevelopmentZone, Gansu University of Chinese Medicine.No.1, Chinese Medicine Road,Lanzhou 730101, Gansu Province, China[5]Department of Thoracic Surgery,Guangdong Provincial People’s Hospital, Guangdong Academy of MedicalSciences, Guangzhou 510080, China[6]Third People’s Hospital of DongguanCity, Dongguan 523326, China[7]Department of Urology, Minimally InvasiveSurgery Center, The First Affiliated Hospital of Guangzhou Medical University,and Guangdong Key Laboratory of Urology, Guangzhou, Guangdong, China
Background Long noncoding RNAs (lncRNAs) are emerging as master regulators for gene expression and thus play a vital role in human tumorigenesis and progression. But the involvement of novel lncRNAs in non-small cell lung cancer (NSCLC) remains largely unelucidated. Methods A total of 170 NSCLC and their adjacent non-tumor tissues were enrolled to detect the expression of Lnc-LSAMP-1 by RT-qPCR. The effects of Lnc-LSAMP-1 on cell proliferation, migration, invasion and drug-sensitivity were determined by in vitro and in vivo experiments. The proteins that interact with Lnc-LSAMP-1were confirmed by RNA pull-down assay. RNA-sequencing were used to identify the potential targets of Lnc-LSAMP-1 in NSCLC. Results We found that Lnc-LSAMP-1 was significantly down-regulated in 170 cases of NSCLC tissues when compared to their adjacent non-cancerous tissues. Loss expression of Lnc-LSAMP-1 was notably correlated with unfavorable prognosis of NSCLC patients. The ectopic expression of Lnc-LSAMP-1 drastically inhibited lung cancer cell proliferation, viability, invasion and migration ability, arrested cell cycle and facilitated apoptosis. Chemotherapy sensitization experiments showed that over-expressed Lnc-LSAMP-1 enhanced the inhibition of cell proliferation induced by TKI. Mechanistically, Lnc-LSAMP-1-LSAMP formed a complex which could protect the degradation of LSAMP gene, and thus exerted crucial roles in NSCLC progression and TKI targeted treatment. Conclusions Consequently, our findings highlight the function and prognostic value of Lnc-LSAMP-1 in NSCLC and provide potential novel therapeutic targets and prognostic biomarkers for patients with NSCLC.
基金：
National Natural Science Foundation of
China 82173609, 81872694, 81673267 (J. Lu), 81872127, 81602289 (F. Qiu),
81273149, 81402753, 81672303 (L. Yang), the National Key R&D Projects
(2016YFC0903700), Local Innovative and Research Teams Project of Guangdong
Pearl River Talents Program 2017BT01S155(J. Lu), Guangdong High
School Young Innovative Talents Project ( 2015KQNCX136), Guangzhou
Science Research Program General Project (201707010123, 201804020023),
Guangzhou Municipal Scientific Research Project (1201630073) and Guangzhou
Science and Technology Program Pearl River Nova projects Grant
201710010049 (L. Yang). Guangdong education bureau Characteristic innovation
project Grants 2015KTSCX116 (L.Yang) and Guangdong Provincial Major
Projects Grants 2014KZDXM046, Yangcheng Scholar Grants 1201541589 (J. Lu)
and Young and middle-aged scientific research backbone training project of
Shenzhen People’s Hospital (SYJCYJ202107).
第一作者机构：[1]The State Key Lab of Respiratory Disease, The First Affiliated Hospital,Guangzhou Medical University, 151 Yanjiangxi Road, Guangzhou 510120,China[2]The School of Public Health, The Institute for Chemical Carcinogenesis,Collaborative Innovation Center for Environmental Toxicity, GuangzhouMedical University, Xinzao, Panyu District, Guangzhou 511436, China[7]Department of Urology, Minimally InvasiveSurgery Center, The First Affiliated Hospital of Guangzhou Medical University,and Guangdong Key Laboratory of Urology, Guangzhou, Guangdong, China
共同第一作者：
通讯作者：
通讯机构：[1]The State Key Lab of Respiratory Disease, The First Affiliated Hospital,Guangzhou Medical University, 151 Yanjiangxi Road, Guangzhou 510120,China[2]The School of Public Health, The Institute for Chemical Carcinogenesis,Collaborative Innovation Center for Environmental Toxicity, GuangzhouMedical University, Xinzao, Panyu District, Guangzhou 511436, China
推荐引用方式(GB/T 7714)：
Wei Gong,Yinyan Li,Jianfeng Xian,et al.Long non-coding RNA LSAMP-1 is down-regulated in non-small cell lung cancer and predicts a poor prognosis[J].CANCER CELL INTERNATIONAL.2022,22(1):doi:10.1186/s12935-022-02592-0.
APA：
Wei Gong,Yinyan Li,Jianfeng Xian,Lei Yang,Yuanyuan Wang...&Fuman Qiu.(2022).Long non-coding RNA LSAMP-1 is down-regulated in non-small cell lung cancer and predicts a poor prognosis.CANCER CELL INTERNATIONAL,22,(1)
MLA：
Wei Gong,et al."Long non-coding RNA LSAMP-1 is down-regulated in non-small cell lung cancer and predicts a poor prognosis".CANCER CELL INTERNATIONAL 22..1(2022)
