详情页 - 广东省中医院知识库

当前位置：首页 > 详情页

Systematic characterization of components of Makyo-kanseki-to granule and serum metabolomics for exploring its protective mechanism against acute lung injury in lipopolysaccharide-induced rats

3| 认领 | 导出 | 链接全文 |

文献详情

资源类型：

WOS体系：

Pubmed体系：

收录情况： ◇ SCIE

作者：

机构： [1]Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang, P. R. China. [2]Key Laboratory of Digitalized Quality Evaluation Technology of Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P. R. China. [3]Clinical Laboratory of Integrative Medicine, The first affiliated hospital of Dalian Medical University, Dalian, P. R. China.

出处：

DOI：

ISSN：

关键词： acute lung injury chemical profile liquid chromatography coupled with mass spectrometry Makyo-kanseki-to granule metabolomics

摘要：

Makyo-kanseki-to has been used for the treatment of pneumonia, becoming a basic formula for coronavirus disease 2019. However, the chemical profile of Makyo-kanseki-to granule and its possible mechanism against acute lung injury from terminal metabolic regulation have been unclear. The aim of this study was to characterize the constituents in Makyo-kanseki-to granule and reveal the potential related mechanism of Makyo-kanseki-to granule treatment for acute lung injury using a rat model of lipopolysaccharide-induced acute lung injury. Totally, 78 constituents were characterized based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Makyo-kanseki-to granule could alleviate acute lung injury through modulating rectal temperature, pulmonary edema, histopathology, and processes of inflammatory and oxidative stress. Twenty-two potential biomarkers in acute lung injury rats were identified by metabolomics based on ultra-performance liquid chromatography coupled with quadrupole exactive high-field mass spectrometry. They were mainly involved in amino acids and glycerophospholipid metabolism, which were regulated by Makyo-kanseki-to granule. The present results not only increase the understanding of the chemical profile and molecular mechanism of Makyo-kanseki-to granule mediated protection against acute lung injury but also provide an experimental basis and new ideas for further development and clinical application of Makyo-kanseki-to granule.© 2022 Wiley-VCH GmbH.

基金：

语种：

WOS：

PubmedID：

中科院(CAS)分区：

出版当年[2022]版：

大类 | 3 区工程技术

小类 | 3 区分析化学 3 区工程：化工

最新[2025]版：

大类 | 3 区工程技术

小类 | 3 区分析化学

JCR分区：

出版当年[2021]版：

Q2 CHEMISTRY, ANALYTICAL

最新[2023]版：

Q2 CHEMISTRY, ANALYTICAL

影响因子： 2.8 最新[2023版] 2.7 最新五年平均 3.614 出版当年[2021版] 2.904 出版当年五年平均 3.645 出版前一年[2020版] 3.1 出版后一年[2022版]

第一作者：

第一作者机构： [1]Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang, P. R. China.

通讯作者：

通讯机构： [1]Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang, P. R. China. [*1]Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, P. R. China.

推荐引用方式(GB/T 7714)：

APA：

MLA：

相关文献

[1]In vivo metabolism study of ginsenoside Re in rat using high-performance liquid chromatography coupled with tandem mass spectrometry [2]Chemical Profiling of Constituents of Smilacis glabrae Using Ultra-high Pressure Liquid Chromatography Coupled with LTQ Orbitrap Mass Spectrometry [3]Chemical Profiling of Constituents of Smilacis glabrae Using Ultra-high Pressure Liquid Chromatography Coupled with LTQ Orbitrap Mass Spectrometry [4]Structural characterization and discrimination of Paris polyphylla var. yunnanensis by a molecular networking strategy coupled with ultra-high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry [5]Application of Ultra-High-Performance Liquid Chromatography Coupled with LTQ-Orbitrap Mass Spectrometry for the Qualitative and Quantitative Analysis of Polygonum multiflorum Thumb. and Its Processed Products [6]The Metabolism Study of Oleraisoquinoline in Rats Using Ultrahigh-performance Liquid Chromatography-electrospray Coupled with Quadrupole Time-of-flight Mass Spectrometry and its Bioactivities [7]Identification of potential anti-inflammatory components in Moutan Cortex by bio-affinity ultrafiltration coupled with ultra-performance liquid chromatography mass spectrometry [8]Quantitative comparative analysis of main isoflavonoids and saponins in the Huangqi-Sanqi herb pair using ultra-high-performance liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry [9]Differential metabolic profiles of ginsenosides in artificial gastric juice using ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry [10]Rapid Characterization and Identification of Flavonoids in Radix Astragali by Ultra-High-Pressure Liquid Chromatography Coupled with Linear Ion Trap-Orbitrap Mass Spectrometry