机构：[1]Department of Laboratory Medicine, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People’s Hospital Guangzhou,[2]Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, PR China大德路总院检验科大德路总院检验科广东省中医院[3]The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, PR China广东省中医院[4]Department of Laboratory Medicine, Guangzhou No.11 People’s Hospital, Guangzhou Cadre Health Management Center, Guangzhou, PR China[5]Department of Laboratory Medicine, The Affiliated Hexian Memorial Hospital of Southern Medical University, Guangzhou, PR China[6]Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, PR China
As an important virulence phenotype of Escherichia coli, the regulation mechanism of biofilm by non-coding RNA and quorum sensing system has not been clarified. Here, by transcriptome sequencing and RT-PCR analysis, we found CsrB, a non-coding RNA of the carbon storage regulation system, was positively regulated by the LuxR protein SdiA. Furthermore, beta-galactosidase reporter assays showed that SdiA enhanced promoter transcriptional activity of csrB. The consistent dynamic expression levels of SdiA and CsrB during Escherichia coli growth were also detected. Moreover, curli assays and biofilm assays showed sdiA deficiency in Escherichia coli SM10 lambda pi or BW25113 led to a decreased formation of biofilm, and was significantly restored by over-expression of CsrB. Interestingly, the regulations of SdiA on CsrB in biofilm formation were enhanced by quorum sensing signal molecules AHLs. In conclusion, SdiA plays a crucial role in Escherichia coli biofilm formation by regulating the expression of non-coding RNA CsrB. Our study provides new insights into SdiA-non-coding RNA regulatory network involved in Escherichia coli biofilm formation.
基金：
National Natural Science Foundation of China [81672081, 82072345, 81801973]; Medical Scientific Research Foundation of Guangdong Province [A2020560]; Guangdong Provincial Hospital of Traditional Chinese Medicine [YN2018QJ01, YN2019QJ03, YN2019MJ01]
第一作者机构：[2]Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, PR China[3]The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, PR China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Laboratory Medicine, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People’s Hospital Guangzhou,[2]Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, PR China[*1]Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou, PR China.
推荐引用方式(GB/T 7714)：
Zhang Shebin,Shu Yurong,Zhang Weizheng,et al.Quorum sensing N-acyl homoserine lactones-SdiA enhances the biofilm formation of E. coli by regulating sRNA CsrB expression[J].HELIYON.2023,9(11):doi:10.1016/j.heliyon.2023.e21658.
APA：
Zhang, Shebin,Shu, Yurong,Zhang, Weizheng,Xu, Zhenjie,Li, Youqiang...&Lu, Yang.(2023).Quorum sensing N-acyl homoserine lactones-SdiA enhances the biofilm formation of E. coli by regulating sRNA CsrB expression.HELIYON,9,(11)
MLA：
Zhang, Shebin,et al."Quorum sensing N-acyl homoserine lactones-SdiA enhances the biofilm formation of E. coli by regulating sRNA CsrB expression".HELIYON 9..11(2023)
