机构：[1]Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China[2]Ocean University of China, Qingdao 266100, China[3]Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, China广东省中医院
Legionella spp. are important waterborne pathogens. Molecular typing has become an important method for outbreaks investigations and source tracking of Legionnaires. In a survey program conducted by the Guangzhou Center for Disease Control and Prevention, multiple serotypes Legionella pneumophila (L. pneumophila) were isolated from waters in air-conditioning cooling towers in urban Guangzhou region, China between 2008 and 2011. Three genotyping methods, mip (macrophage infectivity potentiator) genotyping, SBT (sequence-based typing), and FAFLP (fluorescent amplified fragment length polymorphism analysis) were used to type these waterborne L. pneumophila isolates. The three methods were capable of typing all the 134 isolates and a reference strain of L. pneumophila (ATCC33153), with discriminatory indices of 0.7034, 0.9218, and 0.9376, for the mip, SBT, and FAFLP methods respectively. Among the 9 serotypes of the 134 isolates, 10, 50, and 34 molecular types were detected by the mip, SBT, and FAFLP methods respectively. The mip genotyping and SBT typing are more feasible for inter-laboratory results sharing and comparison of different types of L. pneumophila. The SBT and FAFLP typing methods were rapid with higher discriminatory abilities. Combinations of two or more of the typing methods enables more accurate typing of Legionella isolates for outbreak investigations and source tracking of Legionnaires.
基金：
Project for Key Medicine Discipline Construction of Guangzhou Municipality [2017-2019-07]
第一作者机构：[1]Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China[2]Ocean University of China, Qingdao 266100, China
通讯作者：
通讯机构：[1]Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China[*1]Department of Microbiology, Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
推荐引用方式(GB/T 7714)：
Gong Xiangli,Li Juntao,Zhang Ying,et al.Molecular typing of Legionella pneumophila from air-conditioning cooling waters using mip gene, SBT, and FAFLP methods[J].JOURNAL OF MICROBIOLOGICAL METHODS.2017,139:1-7.doi:10.1016/j.mimet.2017.04.002.
APA：
Gong, Xiangli,Li, Juntao,Zhang, Ying,Hou, Shuiping,Qu, Pinghua...&Chen, Shouyi.(2017).Molecular typing of Legionella pneumophila from air-conditioning cooling waters using mip gene, SBT, and FAFLP methods.JOURNAL OF MICROBIOLOGICAL METHODS,139,
MLA：
Gong, Xiangli,et al."Molecular typing of Legionella pneumophila from air-conditioning cooling waters using mip gene, SBT, and FAFLP methods".JOURNAL OF MICROBIOLOGICAL METHODS 139.(2017):1-7
