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Immunomodulatory activities of phlorizin metabolites in lipopolysaccharide-stimulated RAW264.7 cells

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机构: [1]Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510006, China [2]The Postdoctoral Research Station, Guangzhou University of Chinese Medicine, Guangzhou 510120, China [3]School of Chinese Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou 510120, China
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关键词: Phlorizin metabolites LPS RAW264 7 Immunomodulatory activity

摘要:
The immunomodulatory effects of two new phlorizin metabolites, phloretin 4-O-beta-D-glucuronide (1), 6methoxyl- phloretin-2-O-beta-D-glucuronide (2), together with phloretin-2-O-beta-D-glucuronide (3) on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells were determined. 1-3 (1-5 mg/ml) significantly inhibited the production of NO (p < 0.01). At the concentration of 5 mu g/ml, 2 and 3 further inhibited iNOS mRNA expression (p < 0.01 or 0.05), and 1-3 inhibited iNOS protein expression (p < 0.01). Conversely, they all promoted the proinflammatory cytokine TNF-alpha mRNA expression (p < 0.01). For IL10 mRNA, 1 and 3, which are main metabolism forms in rat plasma, obviously promoted its expression (p < 0.01), while metabolite 2, which was only detected in rat urine, showed inhibitory activity, but 1-3 alone without LPS stimulation had no effect on the expression of both TNF-alpha and IL-10 mRNA expression. 1 further inhibited VEGF, CCL2 and CXCL1 mRNA expression at the concentration of 5-25 mu g/ml (p < 0.01). These results indicated phloretin's metabolites with different structural forms showed different immunomodulatory activities. (C) 2017 Elsevier Masson SAS. All rights reserved.

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出版当年[2016]版:
大类 | 3 区 医学
小类 | 4 区 医学:研究与实验 4 区 药学
最新[2025]版:
大类 | 2 区 医学
小类 | 2 区 医学:研究与实验 2 区 药学
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出版当年[2015]版:
Q3 MEDICINE, RESEARCH & EXPERIMENTAL Q3 PHARMACOLOGY & PHARMACY
最新[2024]版:
Q1 MEDICINE, RESEARCH & EXPERIMENTAL Q1 PHARMACOLOGY & PHARMACY

影响因子: 最新[2024版] 最新五年平均 出版当年[2015版] 出版当年五年平均 出版前一年[2014版] 出版后一年[2016版]

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第一作者机构: [1]Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510006, China [2]The Postdoctoral Research Station, Guangzhou University of Chinese Medicine, Guangzhou 510120, China
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