机构:[1]Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510006, China广东省中医院[2]The Postdoctoral Research Station, Guangzhou University of Chinese Medicine, Guangzhou 510120, China[3]School of Chinese Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou 510120, China
The immunomodulatory effects of two new phlorizin metabolites, phloretin 4-O-beta-D-glucuronide (1), 6methoxyl- phloretin-2-O-beta-D-glucuronide (2), together with phloretin-2-O-beta-D-glucuronide (3) on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells were determined. 1-3 (1-5 mg/ml) significantly inhibited the production of NO (p < 0.01). At the concentration of 5 mu g/ml, 2 and 3 further inhibited iNOS mRNA expression (p < 0.01 or 0.05), and 1-3 inhibited iNOS protein expression (p < 0.01). Conversely, they all promoted the proinflammatory cytokine TNF-alpha mRNA expression (p < 0.01). For IL10 mRNA, 1 and 3, which are main metabolism forms in rat plasma, obviously promoted its expression (p < 0.01), while metabolite 2, which was only detected in rat urine, showed inhibitory activity, but 1-3 alone without LPS stimulation had no effect on the expression of both TNF-alpha and IL-10 mRNA expression. 1 further inhibited VEGF, CCL2 and CXCL1 mRNA expression at the concentration of 5-25 mu g/ml (p < 0.01). These results indicated phloretin's metabolites with different structural forms showed different immunomodulatory activities. (C) 2017 Elsevier Masson SAS. All rights reserved.
基金:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81202888]; Guangdong technology Program [2013B09080 0052]
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外文
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出版当年[2016]版:
大类|3 区医学
小类|4 区医学:研究与实验4 区药学
最新[2025]版:
大类|2 区医学
小类|2 区医学:研究与实验2 区药学
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出版当年[2015]版:
Q3MEDICINE, RESEARCH & EXPERIMENTALQ3PHARMACOLOGY & PHARMACY
最新[2024]版:
Q1MEDICINE, RESEARCH & EXPERIMENTALQ1PHARMACOLOGY & PHARMACY
第一作者机构:[1]Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510006, China[2]The Postdoctoral Research Station, Guangzhou University of Chinese Medicine, Guangzhou 510120, China
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推荐引用方式(GB/T 7714):
Zhao Ya,Liu ChunPing,Lai XiaoPing,et al.Immunomodulatory activities of phlorizin metabolites in lipopolysaccharide-stimulated RAW264.7 cells[J].BIOMEDICINE & PHARMACOTHERAPY.2017,91:49-53.doi:10.1016/j.biopha.2017.04.066.
APA:
Zhao, Ya,Liu, ChunPing,Lai, XiaoPing,Hou, ShaoZhen,Zeng, Xing&Li, Xiong.(2017).Immunomodulatory activities of phlorizin metabolites in lipopolysaccharide-stimulated RAW264.7 cells.BIOMEDICINE & PHARMACOTHERAPY,91,
MLA:
Zhao, Ya,et al."Immunomodulatory activities of phlorizin metabolites in lipopolysaccharide-stimulated RAW264.7 cells".BIOMEDICINE & PHARMACOTHERAPY 91.(2017):49-53