机构：[1]Center for Tuberculosis Control of Guangdong Province, Guangzhou 510630, China[2]Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China[3]Foshan Fourth People's Hospital, Foshan 528000, China[4]Bao'an Chronic Diseases Prevention and Cure Hospital, Shenzhen 518102, China[5]Guangzhou Chest Hospital, Guangzhou 510095, China[6]The Second People's Hospital of Zhongshan City, Zhongshan 528447, China[7]Chinese Medicine Hospital of Huangpu District, Guangzhou 510700, China[8]GuangDong Provincial Hospital of Chinese Medicine, Guangdong 510120, China广东省中医院[9]Chronic Disease Control and Prevention Station of Dongguan, Guangdong 523008, China[10]Department of Microbiology and Immunology, Jinan University, Guangzhou 510623, China[11]College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China
Object: Tuberculosis (TB) continues to be one of the most serious infectious diseases in the world, however, no effective biomarkers can be used for rapid screening of latent tuberculosis infection (LTBI) and active TB. In this study, serum cytokines were screened and tested as potential biomarker for TB diagnosis. Method: Cytokine array was used to track the cytokine profile and its dynamic change after TB infection. The different expressions of cytokines were confirmed by ELISA assay. ROC curve analyses were used to evaluate the efficacy of a cytokine or cytokine combination for diagnosis. Results: Eotaxin-2, ICAM-1, MCSF, IL-12p70, and IL-11 were significantly higher in the LTBI individuals. I-309, MIG, Eotaxin-2, IL-8, ICAM-1, IL-6sR, and Eotaxin were significantly higher in active TB patients. ROC curve analyses gave AUCs of 0.843, 0.898, and 0.888 for 1-309, MIG, and IL-8, respectively, and 0.894 for the combination panel in active TB diagnosis. IFN-gamma/IL-4 and IL-2/TNF-alpha ratios exhibit dynamic changes in the healthy control and LTBI to different stages of active TB. Conclusions: Serum cytokines, including 1-309 and MIG, IL-8, Extoxin-2, ICAM-1 and combinations of cytokines, including IFN-gamma/IL-4 and IL-2/TNF-alpha, can be used as serum biomarkers for LTBI and active TB screening, thus indicating prospective clinical applications. (c) 2016 Elsevier Ltd. All rights reserved.
基金：
National Science and Technology Key Project for Major Infectious Diseases [2012ZX10004903, 2013ZX10003001, 2014ZX10003002]; Guangdong Natural Science FoundationNational Natural Science Foundation of Guangdong Province [WSTJJ201 20319130623198210011211]; Science and Technology of Guangdong Province [2013B051000055]
第一作者机构：[1]Center for Tuberculosis Control of Guangdong Province, Guangzhou 510630, China
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推荐引用方式(GB/T 7714)：
Chen Tao,Li Zhenyan,Yu Li,et al.Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array[J].TUBERCULOSIS.2016,97:108-117.doi:10.1016/j.tube.2015.12.007.
APA：
Chen, Tao,Li, Zhenyan,Yu, Li,Li, Haicheng,Lin, Jinfei...&Zhou, Jie.(2016).Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array.TUBERCULOSIS,97,
MLA：
Chen, Tao,et al."Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array".TUBERCULOSIS 97.(2016):108-117
