机构：[a]The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou510006, China广东省中医院[b]Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510006, China[c]Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology,Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai 201203, China
Extraction optimization, purification, characterization, sulfation and antitumor activity of polysaccharides from the fruit body of Borojoa sorbilis cuter were investigated in present study. The optimal Ultrahigh Pressure extraction condition was determined as: extraction once with the solid-liquid ratio of 1:10 in 30 degrees C and 1500 Mpa for crude polysaccharide (BP) and experimental yield was 8.28%. Four water-soluble polysaccharides named as BP1-1, BP1-2, BP1-3 and BP1-4, with molecular weight of 35.8, 32.4, 30.1 and 27.7 kDa, were purified by DEAE Sepharose and Superdex 200 chromatography. On the basis of chemical and spectroscopic analyses, BP1-1-BP1-4 were found to be neutral beta-D-galactan containing a (1 -> 4) linked backbone. S-BP1s with the DSS of 1.18, was sulfated by chloro-sulfonic acid-pyridine method. Furthermore, S-BP1s exhibited significant in vitro antitumor activity against liver cancer HepG2 and lung cancer A549 cells in a dose-dependent manner. The results indicated that S-BP1s could be potentially developed as functional antitumor drug. (C) 2016 Elsevier Ltd. All rights reserved.
基金：
This work was supported by the National Natural Science Foun-dation of China (No. 81202398), Guangdong provincial scienceand technology project (Nos. 2013B010102006, 2014A020221035,and 2015A020211025), the Specific Research Project of Scienceand Technology of Guangdong Provincial Hospital of TCM (Nos.YN2014ZHR209 and YN2015MS03).
第一作者机构：[a]The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou510006, China
共同第一作者：
通讯作者：
通讯机构：[a]The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou510006, China[c]Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology,Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai 201203, China[*1]The Second Affiliated Hospital of Guangzhou Uni-versity of Chinese Medicine, Guangdong Provincial Academy of Chinese MedicalSciences, Guangzhou 510006, China.
推荐引用方式(GB/T 7714)：
Fangfang Xu,Kangsheng Liao,Yunshan Wu,et al.Optimization, characterization, sulfation and antitumor activity of neutral polysaccharides from the fruit of Borojoa sorbilis cuter[J].CARBOHYDRATE POLYMERS.2016,151:364-372.doi:10.1016/j.carbpol.2016.05.091.
APA：
Fangfang Xu,Kangsheng Liao,Yunshan Wu,Qi Pan,Lilan Wu...&Bo Liu.(2016).Optimization, characterization, sulfation and antitumor activity of neutral polysaccharides from the fruit of Borojoa sorbilis cuter.CARBOHYDRATE POLYMERS,151,
MLA：
Fangfang Xu,et al."Optimization, characterization, sulfation and antitumor activity of neutral polysaccharides from the fruit of Borojoa sorbilis cuter".CARBOHYDRATE POLYMERS 151.(2016):364-372
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