机构：[a]Department of Nephrology and Rheumatology, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, PR China[b]Institute of Integrated Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai, PR China[c]Department of Nephrology, Zhejiang Hospital, Hangzhou, PR China[d]Nephrology Center, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, PR China广东省中医院
Accumulating evidence suggests that inflammatory processes are involved in the development of diabetic nephropathy (DN). However, there are no effective interventions for inflammation in the diabetic kidneys. Here, we tested the hypothesis that Astragaloside IV(AS-IV), a novel saponin purified from Astragalus membranaceus (Fisch) Bge, ameliorates DN in streptozotocin (STZ)-induced diabetic rats through anti-inflammatory mechanisms. Diabetes was induced with STZ (65 mg/kg) by intraperitoneal injection in rats. Two weeks after STZ injection, rats were divided into three groups (n = 8/each group), namely, diabetic rats, diabetic rats treated with AS-IV at 5 and 10 mg kg(-1) d(-1), p.o., for 8 weeks. The normal rats were chosen as nondiabetic control group (n = 8). The rats were sacrificed 10 weeks after induction of diabetes. AS-IV ameliorated albuminuria, renal histopathology and podocyte foot process effacement in diabetic rats. Renal NF-kappa B activity, as wells as protein and mRNA expression were increased in diabetic kidneys, accompanied by an increase in mRNA expression and protein content of TNF-alpha, MCP-1 and ICAM-1 in kidney tissues. The alpha(1)-chain type IV collagen mRNA was elevated in the kidneys of diabetic rats. All of these abnormalities were partially restored by AS-IV. AS-IV also decreased the serum levels of TNF-alpha, MCP-1 and ICAM-1 in diabetic rats. These findings suggest that AS-IV, a novel anti-inflammatory agent, attenuated DN in rats through inhibiting NE-kappa B mediated inflammatory genes expression. (C) 2013 Elsevier Ltd. All rights reserved.
基金：
This work was supported by research Grant from National Natural
Science Foundation of China (No. 81000305 to Dingkun Gui).
第一作者机构：[a]Department of Nephrology and Rheumatology, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, PR China
共同第一作者：
通讯作者：
通讯机构：[a]Department of Nephrology and Rheumatology, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, PR China[d]Nephrology Center, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, PR China[*1]No. 600 Yishan Road, Shanghai, PR China.
推荐引用方式(GB/T 7714)：
Dingkun Gui,Jianhua Huang,Yongping Guo,et al.Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression[J].CYTOKINE.2013,61(3):970-977.doi:10.1016/j.cyto.2013.01.008.
APA：
Dingkun Gui,Jianhua Huang,Yongping Guo,Jianguo Chen,Yifang Chen...&Niansong Wang.(2013).Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression.CYTOKINE,61,(3)
MLA：
Dingkun Gui,et al."Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression".CYTOKINE 61..3(2013):970-977
