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α-glucosidase inhibitory effect and simultaneous quantification of three major flavonoid glycosides in microctis folium(Open Access)

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机构: [a]State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau [b]Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China [c]Guangdong Research Institute of Traditional Chinese Medicine, Guangzhou 510520, China
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关键词: Microctis Folium flavonoid glycosides α-glucosidase inhibitory effect HPLC-DAD quantification

摘要:
Microctis Folium, the leaves of Microcos paniculata L., is a commonly used herbal tea material. The methanol extract of Microctis Folium and its principle compounds vitexin (1), isovitexin (2) and isorhamnetin 3-O-β-D-rutinoside (3) were investigated for their α-glucosidase inhibitory effects. The extract showed strong α-glucosidase inhibitory effect (IC50 = 61.30 μg/mL) and the three flavonoid glycosides 1-3 exerted satisfactory α-glucosidase inhibitory effects, with IC50 values of 244.0 μM, 266.2 μM and 275.4 μM, respectively. A simple and reliable HPLC-DAD method was developed for the quantification of the three flavonoid glycosides in Microctis Folium and applied successfully to determine contents of these components in samples collected from different locations. This study suggested that Microctis Folium may be a promising candidate for development of herbal antidiabetes drugs, and vitexin, isovitexin and isorhamnetin 3-O-β-D-rutinoside can be the biomarkers and chemical markers for this plant substance. © 2013 by the authors.

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出版当年[2012]版:
大类 | 4 区 化学
小类 | 4 区 有机化学
最新[2025]版:
大类 | 3 区 化学
小类 | 3 区 生化与分子生物学 3 区 化学:综合
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第一作者机构: [a]State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau
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