机构：[1]Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.[2]Department of Otorhinolaryngology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.[3]Department of Anesthesiology, Zhongshan Hospital of Traditional Chinese Medicine, Affiliated to Guangzhou University of Chinese Medicine, Zhongshan, Guangdong Province, China.
Adenoid cystic carcinoma (ACC) is one of the most frequent malignancies of salivary glands. The objective of this study was to identify key genes and potential mechanisms during ACC samples.The gene expression profiles of GSE88804 data set were downloaded from Gene Expression Omnibus. The GSE88804 data set contained 22 samples, including 15 ACC samples and 7 normal salivary gland tissues. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were constructed, and protein-protein interaction network of differentially expressed genes (DEGs) was performed by Cytoscape. The top 10 hub genes were analyzed based on Gene Expression Profiling Interactive Analysis. Then, DEGs between ACC samples and normal salivary gland samples were analyzed by gene set enrichment analysis. Furthermore, miRTarBase and Cytoscape were used for visualization of miRNA-mRNA regulatory network. KEGG pathway analysis was undertaken using DIANA-miRPath v3.0.In total, 382 DEGs were identified, including 119 upregulated genes and 263 downregulated genes. GO analysis showed that DEGs were mainly enriched in extracellular matrix organization, extracellular matrix, and calcium ion binding. KEGG pathway analysis showed that DEGs were mainly enriched in p53 signaling pathway and salivary secretion. Expression analysis and survival analysis showed that ANLN, CCNB2, CDK1, CENPF, DTL, KIF11, and TOP2A are all highly expressed, which all may be related to poor overall survival. Predicted miRNAs of 7 hub DEGs mainly enriched in proteoglycans in cancer and pathways in cancer.This study indicated that identified DEGs and hub genes might promote our understanding of molecular mechanisms, which might be used as molecular targets or diagnostic biomarkers for ACC.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|4 区医学
小类|3 区医学：内科
最新[2025]版：
大类|4 区医学
小类|4 区医学：内科
第一作者：
第一作者机构：[1]Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.[*1]Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Nanchang University, No 1, Minde Road, Nanchang, Jiangxi 330006, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.[*1]Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Nanchang University, No 1, Minde Road, Nanchang, Jiangxi 330006, China
推荐引用方式(GB/T 7714)：
Liu Hong-Bing,Huang Guan-Jiang,Luo Meng-Si.Transcriptome analyses identify hub genes and potential mechanisms in adenoid cystic carcinoma.[J].Medicine.2020,99(2):e18676.doi:10.1097/MD.0000000000018676.
APA：
Liu Hong-Bing,Huang Guan-Jiang&Luo Meng-Si.(2020).Transcriptome analyses identify hub genes and potential mechanisms in adenoid cystic carcinoma..Medicine,99,(2)
MLA：
Liu Hong-Bing,et al."Transcriptome analyses identify hub genes and potential mechanisms in adenoid cystic carcinoma.".Medicine 99..2(2020):e18676
