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Anticancer activity of sugiol against ovarian cancer cell line SKOV3 involves mitochondrial apoptosis, cell cycle arrest and blocking of the RAF/MEK/ERK signalling pathway.

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机构： [1]Department of Gynaecology, Hubei Maternity and Child Health Hospital, Wuhan, China [2]Department of Obstetrics and Gynaecology, Beijing Hospital, Beijing, China [3]Department of Obstetrics and Gynaecology, Haidian Woman’s and Children’s Hospital, Beijing, China [4]Department of Obstetrics and Gynaecology, Dongguan Hospital of Traditional Chinese Medicine, Dongguan Guangdong, China

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关键词： ovarian cancer cell cycle arrest apoptosis RAF/MEK/ERK cell migration

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Ovarian cancer is one of the leading causes of cancer-related deaths in women. Treatments for ovarian cancer include surgery followed by chemotherapy. However, the survival rate for ovarian cancer is still not satisfactory. Moreover, the current chemotherapy has numerous associated side effects. Therefore there is an urgent need to look for novel and more viable treatment options. Against this backdrop the present study was designed to evaluate the anticancer activity of sugiol against ovarian cancer cells. Cell viability was assessed by CCK8 assay, apoptosis by DAPI, AO/ER and annexin V/PI staining. Mitochondrial membrane potential and cell cycle analysis was performed by flow cytometry. Cell migration was investigated by wound healing assay. Protein expression was monitored by western blotting. The results of the present study indicated that sugiol exerts significant (p < 0.0001) anticancer effects on SKOV3 cancer cells with an IC50 of 25 μM. However, sugiol exhibited less cytotoxicity against normal ovarian cells with an IC50 of 62.5 μM. The anticancer effects of sugiol were found to be due to G0/G1 cell cycle arrest and mitochondrial apoptosis. Sugiol also inhibited cell migration of SKOV3 cells dose dependently. Moreover, the results showed that sugiol could inhibit the RAF/MEK/ERK signalling pathway in a dose-dependent manner. The results of the present study indicate that sugiol exerts potent anticancer effects on SKOV3 cells via induction of cell cycle arrest, mitochondrial apoptosis and inhibition of the RAF/MEK/ERK signalling pathway. Copyright: © 2017 Termedia & Banach.

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出版当年[2019]版：

大类 | 4 区医学

小类 | 3 区医学：内科

最新[2025]版：

大类 | 4 区医学

小类 | 3 区医学：内科

第一作者：

第一作者机构： [1]Department of Gynaecology, Hubei Maternity and Child Health Hospital, Wuhan, China

通讯作者：

通讯机构： [2]Department of Obstetrics and Gynaecology, Beijing Hospital, Beijing, China [*1]Department of Obstetrics and Gynaecology, Beijing Hospital, 1 Dongdan Dahua Road, 100730 Beijing, China

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