机构：[a]Department of Oncology, Hebei General Hospital, Shijiazhuang, 050051, Hebei, China[b]Department of Neurosurgery, Hebei General Hospital, Shijiazhuang, 050051, Hebei, China[c]Department of Nursing, Hebei University of Chinese Medicine, Shijiazhuang, 050020, Hebei, China[d]The Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Institute of Urology of Shenzhen PKUHKUSTMedical Center, Shenzhen, 518036, Guangdong, China北京大学深圳医院深圳医学信息中心香港大学深圳医院[e]Graduate School, Hebei Medical University, Shijiazhuang, 050051, Hebei, China
The histone demethylase KDM6B, also known as jumonji domain-containing protein 3 (JMJD3), is an epigenetic regulator which plays important roles in immune activation, tissue regeneration, cellular senescence and cancer metastasis. But, the role of KDM6B in glioma metastasis is poorly understood. In this study, we achieved transcriptional regulation of KDM6B in glioma cells using CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa). Our results showed that KDM6B promotes the proliferation, migration and invasion of human glioblastoma cells U87 and U251 using CCK8, scratch and transwell assays. Further results indicated that KDM6B increases the expression of SNAI1, a key factor of epithelial-mesenchymal transition (EMT). KDM6B catalyzes the demethylation of histone H3 Lys 27 trimethylation (H3K27me3) in the promoter of SNAI1, which is important for SNAI1 upregulation. Taken together, these findings provide new insight into the mechanism by which KDM6B promotes glioma metastasis.
基金：
This work was supported by Beijing nonprofit foundation for medicine and health (No. YWJKJJHKYJJ-F1174B), and the Natural Science Foundation of Hebei Province (No H2016423023).
第一作者机构：[a]Department of Oncology, Hebei General Hospital, Shijiazhuang, 050051, Hebei, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Aixia Sui,Yongbing Xu,Junjie Yang,et al.The histone H3 Lys 27 demethylase KDM6B promotes migration and invasion of glioma cells partly by regulating the expression of SNAI1.[J].NEUROCHEMISTRY INTERNATIONAL.2019,124:123-129.doi:10.1016/j.neuint.2019.01.006.
APA：
Aixia Sui,Yongbing Xu,Junjie Yang,Baogen Pan,Jiang Wu...&Xiaoqiang Guo.(2019).The histone H3 Lys 27 demethylase KDM6B promotes migration and invasion of glioma cells partly by regulating the expression of SNAI1..NEUROCHEMISTRY INTERNATIONAL,124,
MLA：
Aixia Sui,et al."The histone H3 Lys 27 demethylase KDM6B promotes migration and invasion of glioma cells partly by regulating the expression of SNAI1.".NEUROCHEMISTRY INTERNATIONAL 124.(2019):123-129
