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Kaempferol inhibits the migration and invasion of rheumatoid arthritis fibroblast-like synoviocytes by blocking activation of the MAPK pathway.

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资源类型：
Pubmed体系：
作者：
Pan Dongmei[1] * ; Li Nan[2] * ; Liu Yanyan[3] * ; Xu Qiang[4] ; Liu Qingping[4] ; You Yanting[3] ; Wei Zhenquan[1] ; Jiang Yubao[4] ; Liu Minying[4] ; Guo Tianfeng[1] ; Cai Xudong[1] ; Liu Xiaobao[1] ; Wang Qiang[1] ; Liu Mingling[4] ; Lei Xujie[4] ; Zhang Mingying[4] ; Zhao Xiaoshan[3,*1] ; Lin Changsong[4,*2] ;
机构： [1]Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China [2]School of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China [3]School of Traditional Chinese Medicine, the Southern Medical University, Guangzhou, Guangdong, China [4]Department of Rheumatology, the First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China
出处：
DOI： 10.1016/j.intimp.2017.12.011
ISSN：

关键词： Kaempferol Rheumatoid arthritis Migration Invasion Fibroblast-like synoviocyte MAPK

摘要：
In rheumatoid arthritis (RA), fibroblast-like synoviocytes (FLSs) play an essential role in cartilage destruction. Aggressive migration and invasion by FLSs significantly affect RA pathology. Kaempferol has been shown to inhibit cancer cell migration and invasion. However, the effects of kaempferol on RA FLSs have not been investigated. Our study aimed to determine the effects of kaempferol on RA both in vitro and in vivo. In vitro, cell migration and invasion were measured using scratch assays and the Boyden chamber method, respectively. The cytoskeletal reorganization of RA FLSs was evaluated by immunofluorescence staining. Matrix metalloproteinase (MMP) levels were measured by real-time PCR, and protein expression levels were measured by western blotting. In vivo, the effects of kaempferol were evaluated in mice with CIA. The results showed that kaempferol reduced migration, invasion and MMP expression in RA FLSs. In addition, we demonstrated that kaempferol inhibited reorganization of the actin cytoskeleton during cell migration. Moreover, kaempferol dramatically suppressed tumor necrosis factor (TNF)-α-induced MAPK activation without affecting the expression of TNF-α receptors. We also demonstrated that kaempferol attenuated the severity of arthritis in mice with CIA. Taken together, these results suggested that kaempferol inhibits the migration and invasion of FLSs in RA by blocking MAPK pathway activation without affecting the expression of TNF-α receptors. Copyright © 2017 Elsevier B.V. All rights reserved.

基金：
National Natural Science Foundation of China (no 81573930), Guangdong Natural Science Foundation (no 2014A030313403) and Guangdong Province High Level University Construction Foundation (no AFD018171Z11056).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类 | 3 区 医学
小类 | 3 区 免疫学 3 区 药学
最新[2025]版：
大类 | 2 区 医学
小类 | 2 区 药学 3 区 免疫学
第一作者：
第一作者机构： [1]Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China
共同第一作者：
通讯作者：
通讯机构： [3]School of Traditional Chinese Medicine, the Southern Medical University, Guangzhou, Guangdong, China [4]Department of Rheumatology, the First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China [*1]School of Traditional Chinese Medicine, the Southern Medical University, No. 1023-1063 Shatai Road, Guangzhou, Guangdong 510515, China [*2]Department of Rheumatology, the First Affiliated Hospital, Guangzhou University of Chinese Medicine, No. 16 Jichang Road, Guangzhou, Guangdong 510405, China.
推荐引用方式(GB/T 7714)：
Pan Dongmei,Li Nan,Liu Yanyan,et al.Kaempferol inhibits the migration and invasion of rheumatoid arthritis fibroblast-like synoviocytes by blocking activation of the MAPK pathway.[J].International immunopharmacology.2018,55:174-182.doi:10.1016/j.intimp.2017.12.011.
APA：
Pan Dongmei,Li Nan,Liu Yanyan,Xu Qiang,Liu Qingping...&Lin Changsong.(2018).Kaempferol inhibits the migration and invasion of rheumatoid arthritis fibroblast-like synoviocytes by blocking activation of the MAPK pathway..International immunopharmacology,55,
MLA：
Pan Dongmei,et al."Kaempferol inhibits the migration and invasion of rheumatoid arthritis fibroblast-like synoviocytes by blocking activation of the MAPK pathway.".International immunopharmacology 55.(2018):174-182

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