详情页 - 广东省中医院知识库

当前位置：首页 > 详情页

Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate.

文献详情

资源类型：

Pubmed体系：

作者：

机构： [1]CAS Key Laboratory of Tropical Marine Bio‐Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China [2]Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China [3]Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China [4]South China Sea Bio‐Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, Guangdong, China

出处：

DOI：

ISSN：

关键词： ethyl (R)‐3‐hydroxybutyrate kinetic resolution novel esterase opposite enantio‐selectivity process optimization

摘要：

The two enantiomers of ethyl 3-hydroxybutyrate are important intermediates for the synthesis of a great variety of valuable chiral drugs. The preparation of chiral drug intermediates through kinetic resolution reactions catalyzed by esterases/lipases has been demonstrated to be an efficient and environmentally friendly method. We previously functionally characterized microbial esterase PHE21 and used PHE21 as a biocatalyst to generate optically pure ethyl (S)-3-hydroxybutyrate. Herein, we also functionally characterized one novel salt-tolerant microbial esterase WDEst17 from the genome of Dactylosporangium aurantiacum subsp. Hamdenensis NRRL 18085. Esterase WDEst17 was further developed as an efficient biocatalyst to generate (R)-3-hydroxybutyrate, an important chiral drug intermediate, with the enantiomeric excess being 99% and the conversion rate being 65.05%, respectively, after process optimization. Notably, the enantio-selectivity of esterase WDEst17 was opposite than that of esterase PHE21. The identification of esterases WDEst17 and PHE21 through genome mining of microorganisms provides useful biocatalysts for the preparation of valuable chiral drug intermediates. © 2018 Wiley Periodicals, Inc.

基金：

语种：

PubmedID：

中科院(CAS)分区：

出版当年[2017]版：

大类 | 4 区医学

小类 | 4 区分析化学 4 区药物化学 4 区有机化学 4 区药学

最新[2025]版：

大类 | 3 区化学

小类 | 3 区有机化学 4 区分析化学 4 区药物化学 4 区药学

第一作者：

第一作者机构： [1]CAS Key Laboratory of Tropical Marine Bio‐Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China [2]Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China

通讯作者：

通讯机构： [1]CAS Key Laboratory of Tropical Marine Bio‐Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China [2]Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China [4]South China Sea Bio‐Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, Guangdong, China [*1]CAS Key Laboratory of Tropical Marine Bio‐Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, China.

推荐引用方式(GB/T 7714)：

APA：

MLA：

相关文献

[1]Utilization of deep-sea microbial esterase PHE21 to generate chiral sec-butyl acetate through kinetic resolutions. [2]Asymmetric Synthesis of Dihydronaphthalene-1,4-diones via Carbene-Catalyzed Stereodivergent Reaction [3]Organocatalyzed Kinetic Resolution of α-Functionalized Ketones: The Malonate Unit Leads the Way [4]Access to enantioenriched compounds bearing challenging tetrasubstituted stereocenters via kinetic resolution of auxiliary adjacent alcohols. [5]Dynamic Kinetic Resolution of β-Substituted α-Diketones via Asymmetric Transfer Hydrogenation [6]Access to enantioenriched molecules with diverse fluorinated tetrasubstituted stereocenters using hydroxy as a kinetic resolution auxiliary group [7]均匀设计法优选巴戟天盐制工艺及盐制前后5种糖含量比较研究 [8]新颖深海微生物酯酶EstC11的酶学性质及其在手性拆分乙酸苏合香酯中的应用 [9]硅藻土吸附固定化Bacillus sp.DL-2胞内蛋白酶拆分制备(S)-乙酸苏合香酯 [10]响应面法优化岭南炮天雄生物碱提取工艺