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Utilization of deep-sea microbial esterase PHE21 to generate chiral sec-butyl acetate through kinetic resolutions.

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机构： [1]CAS Key Laboratory of Tropical Marine Bio‐Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China [2]Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China [3]Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China [4]South China Sea Bio‐Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, Guangdong, China

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关键词： biocatalysis chiral sec‐butyl acetate deep‐sea microorganism kinetic resolution novel microbial esterase

摘要：

We previously identified and characterized 1 novel deep-sea microbial esterase PHE21 and used PHE21 as a green biocatalyst to generate chiral ethyl (S)-3-hydroxybutyrate, 1 key chiral chemical, with high enantiomeric excess and yield through kinetic resolution. Herein, we further explored the potential of esterase PHE21 in the enantioselective preparation of secondary butanol, which was hard to be resolved by lipases/esterases. Despite the fact that chiral secondary butanols and their ester derivatives were hard to prepare, esterase PHE21 was used as a green biocatalyst in the generation of (S)-sec-butyl acetate through hydrolytic reactions and the enantiomeric excess, and the conversion of (S)-sec-butyl acetate reached 98% and 52%, respectively, after process optimization. Esterase PHE21 was also used to generate (R)-sec-butyl acetate through asymmetric transesterification reactions, and the enantiomeric excess and conversion of (R)-sec-butyl acetate reached 64% and 43%, respectively, after process optimization. Deep-sea microbial esterase PHE21 was characterized to be a useful biocatalyst in the kinetic resolution of secondary butanol and other valuable chiral secondary alcohols. © 2018 Wiley Periodicals, Inc.

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出版当年[2017]版：

大类 | 4 区医学

小类 | 4 区分析化学 4 区药物化学 4 区有机化学 4 区药学

最新[2025]版：

大类 | 3 区化学

小类 | 3 区有机化学 4 区分析化学 4 区药物化学 4 区药学

第一作者：

第一作者机构： [1]CAS Key Laboratory of Tropical Marine Bio‐Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China [2]Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China

通讯作者：

通讯机构： [1]CAS Key Laboratory of Tropical Marine Bio‐Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China [2]Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China [4]South China Sea Bio‐Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, Guangdong, China [*1]CAS Key Laboratory of Tropical Marine Bio‐Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, Guangdong, China.

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Utilization of deep-sea microbial esterase PHE21 to generate chiral sec-butyl acetate through kinetic resolutions.

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