Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro.
机构：[1]Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510000[2]Guangdong Women and Children Hospital, Guangzhou 510000[3]Pediatric Center of Zhujiang Hospital, Southern Medical University, Guangzhou 510000[4]Department of Neurology, Liuzhou Hospital of Traditional Chinese Medicine, Guangxi 545001[5]Department of Blood Transfusion Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510000, P.R. China
Glia maturation factor γ (GMFG) functions to reorganize the actin cytoskeleton and appears to play a causative role in cell migration and adherence. The present study assessed GMFG expression in colorectal cancer cells and tissue specimens and then explored the role of GMFG in colorectal cancer progression in vitro. GMFG protein was highly expressed in colorectal cancer tissues and a metastatic colon cancer cell line. Knockdown of GMFG expression using GMFG siRNA or anti-GMFG antibody decreased the capacity of colon cancer LoVo cell migration and invasion in vitro, while recombinant GMFG treatment induced LoVo cell migration. Furthermore, GMFG knockdown also decreased expression of MMP2 protein and reversed epithelial-mesenchymal transition (EMT) phenotypes in LoVo cells. Co-culture of LoVo cells with human umbilical vein endothelial cells (HUVECs) and exogenous GMFG treatment promoted LoVo cell migration and invasion. The data from the present study indicate that GMFG should be further evaluated as a biomarker for detection of colorectal cancer metastasis and that the targeting of GMFG expression or function could be a novel strategy in the future control of colorectal cancer.
第一作者机构：[1]Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510000[2]Guangdong Women and Children Hospital, Guangzhou 510000
通讯作者：
通讯机构：[1]Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510000[*1]Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, 1838 Baiyun Avenue North, Guangzhou 510000, P.R. China
推荐引用方式(GB/T 7714)：
Wang Huili,Chen Zhijiang,Chang Hongen,et al.Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro.[J].ONCOLOGY REPORTS.2017,37(2):929-936.doi:10.3892/or.2017.5361.
APA：
Wang Huili,Chen Zhijiang,Chang Hongen,Mu Xiaoping,Deng Wenyu...&Wu Bingyi.(2017).Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro..ONCOLOGY REPORTS,37,(2)
MLA：
Wang Huili,et al."Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro.".ONCOLOGY REPORTS 37..2(2017):929-936
