Salvianolic acid B attenuates oxidized low-density lipoprotein-induced endothelial cell apoptosis through inhibition of oxidative stress, p53, and caspase-3 pathways.
机构：[1]Key Laboratory of Molecular Biology, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou (510515), China[2]Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou (510515), China[3]The Fifth Department of Internal Medicine, Dongguan Hospital of Traditional Chinese Medicine, Dongguan, Guangdong Province (523000), China[4]Department of Parasitology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou (510515), China[5]Department of Traditional Chinese Medicine, Shantou Central Hospital, Shantou, Guangdong Province (515031), China[6]Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou (510280), China
To investigate the effect of salvianolic acid B (Sal B) on oxidized low-density lipoprotein (ox-LDL)-induced human umbilical vein endothelial cells (HUVECs) apoptosis and the possible mechanism.
HUVECs were divided into 6 groups, including control group, ox-LDL group, vitamin C group (positive control), and 5, 10 and 20 μg/mL Sal B groups. Cell viability of HUVECs was determined by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The anti-apoptotic effect of Sal B was tested by Hoechst 33258 staining and Annexin V/propidium iodide flflow cytometry analysis. Apoptosis-related genes (p53, Bcl-2 and Bax) expression and caspase-3 activity were also determined. Oxidative stress markers malondialdehyde (MDA) and superoxide dismutase (SOD) were determined by the corresponding kits.
In HUVECs, ox-LDL signifificantly reduced cell viability and induced apoptosis (P<0.05 or P<0.01), however, Sal B diminished the effects of ox-LDL in a dose-dependent manner (P<0.05). Moreover, 10 and 20 μg/mL Sal B reduced the expression levels of p53, increased the Bcl-2/Bax ratio and inhibited the caspase-3 activity in ox-LDL-treated HUVECs (P<0.05). In addition, 5, 10 and 20 μg/mL Sal B signifificantly enhanced the activity of SOD, while decreased the level of MDA in the HUVECs which treated with ox-LDL (P<0.05).
Sal B exhibited anti-apoptotic effects in ox-LDL-induced endothelial cell injury by suppressing oxidative stress, p53, and caspase-3.
基金：
Supported by the National Natural Science Foundation of
China (Nos. 30973850, 81173459, and 81202841) and Planned
Science and Technology Project of Guangdong Province (No.
2010B060500009), China
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|4 区医学
小类|4 区全科医学与补充医学
最新[2025]版：
大类|4 区医学
小类|4 区全科医学与补充医学
第一作者：
第一作者机构：[1]Key Laboratory of Molecular Biology, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou (510515), China[2]Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou (510515), China
通讯作者：
通讯机构：[1]Key Laboratory of Molecular Biology, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou (510515), China[2]Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou (510515), China
推荐引用方式(GB/T 7714)：
Chen Hong-Mei,Luo Hao,Zeng Wen-Bi,et al.Salvianolic acid B attenuates oxidized low-density lipoprotein-induced endothelial cell apoptosis through inhibition of oxidative stress, p53, and caspase-3 pathways.[J].Chinese Journal of Integrative Medicine.2017,doi:10.1007/s11655-016-2645-4.
APA：
Chen Hong-Mei,Luo Hao,Zeng Wen-Bi,Liu Bin,Huang Jia-Cheng...&Zhou Ying-Chun.(2017).Salvianolic acid B attenuates oxidized low-density lipoprotein-induced endothelial cell apoptosis through inhibition of oxidative stress, p53, and caspase-3 pathways..Chinese Journal of Integrative Medicine,,
MLA：
Chen Hong-Mei,et al."Salvianolic acid B attenuates oxidized low-density lipoprotein-induced endothelial cell apoptosis through inhibition of oxidative stress, p53, and caspase-3 pathways.".Chinese Journal of Integrative Medicine .(2017)
