Icariin improves osteoporosis, inhibits the expression of PPARγ, C/EBPα, FABP4 mRNA, N1ICD and jagged1 proteins, and increases Notch2 mRNA in ovariectomized rats.
机构：[1]Department of Traditional Chinese Pharmacology, College of Pharmacy, Jinan University[2]Department of Traditional Chinese Medicine, First Affiliated Hospital, Jinan University, Guangzhou, Guangdong 510632, P.R. China
Icariin (ICA) is a pharmacologically active flavonoid glycoside that shows promise in the treatment and prevention of osteoporosis (OP). However, the mechanisms underlying the anti-osteoporotic effects of ICA remain largely unclear. The present study used quantitative polymerase chain reaction, western blot and immunohistochemical analysis to examine the effects of ICA on several key targets in the Notch signaling pathway in bone tissue in ovariectomized rats. It was observed that ICA has a pronounced beneficial effect on OP rats and inhibits the expression of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα) and fatty acid-binding protein 4 (FABP4) mRNA. In addition, it was identified that ICA downregulates the expression of notch1 intracellular domain (N1ICD) and Jagged1 proteins in bone tissue, and suppresses the effect of N1ICD on Notch2 mRNA expression. It is proposed that ICA inhibits the differentiation of mesenchymal stem cells into adipocytes by inhibiting the expression of PPARγ, C/EBPα and FABP4 mRNA via the Notch signaling pathway. In addition, it is proposed that ICA inhibits the expression of Notch2 mRNA by suppressing the effect of N1ICD. In conclusion, the results provide further mechanistic evidence for the clinical efficacy of ICA in the treatment of OP.
基金：
the National Natural Science Foundation of China (grant nos. 81173619 and 81473509) and Natural Science Foundation of Guangdong Province, China (grant no. S2012040007531).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|4 区医学
小类|4 区医学：研究与实验
最新[2025]版：
大类|4 区医学
小类|4 区医学：研究与实验
第一作者：
第一作者机构：[1]Department of Traditional Chinese Pharmacology, College of Pharmacy, Jinan University
共同第一作者：
通讯作者：
通讯机构：[1]Department of Traditional Chinese Pharmacology, College of Pharmacy, Jinan University[*1]Department of Traditional Chinese Pharmacology, College of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou, Guangdong 510632, P.R. China
推荐引用方式(GB/T 7714)：
Liu Hengrui,Xiong Yingquan,Zhu Xiaofeng,et al.Icariin improves osteoporosis, inhibits the expression of PPARγ, C/EBPα, FABP4 mRNA, N1ICD and jagged1 proteins, and increases Notch2 mRNA in ovariectomized rats.[J].Experimental and therapeutic medicine.2017,13(4):1360-1368.doi:10.3892/etm.2017.4128.
APA：
Liu Hengrui,Xiong Yingquan,Zhu Xiaofeng,Gao Han,Yin Sujuan...&Yang Li.(2017).Icariin improves osteoporosis, inhibits the expression of PPARγ, C/EBPα, FABP4 mRNA, N1ICD and jagged1 proteins, and increases Notch2 mRNA in ovariectomized rats..Experimental and therapeutic medicine,13,(4)
MLA：
Liu Hengrui,et al."Icariin improves osteoporosis, inhibits the expression of PPARγ, C/EBPα, FABP4 mRNA, N1ICD and jagged1 proteins, and increases Notch2 mRNA in ovariectomized rats.".Experimental and therapeutic medicine 13..4(2017):1360-1368
