机构：[1]Department of Oncology, Shunde TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Foshan, Guangdong, China.[2]Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China
Meningioma is one of the common brain tumors in adults. It had been shown that the allopregnanolone biosynthesis was associated with tumorigenesis and PK11195, the translocator protein 18 KDa (TSPO) antagonist, had the effects of the allopregnanolone biosynthesis. However, little is known about the association between the effects of PK11195 on meningioma and the allopregnanolone biosynthesis. To evaluate this, the meningioma cell line IOMM-LEE was applied. Cell viability and proliferation were determined by CCK-8 assay. The IC50 of PK11195 on the IOMM-LEE was 1.505 ± 0.08 nM. The cell viability and proliferation of AC-5216 (TSPO selective ligand, 2 and 4 nM) was blocked by PK11195 (1.5 nM). Further, we evaluated the role of allopregnanolone biosynthesis in the effects of TSPO on meningioma. Enzyme-Linked ImmunoSorbent Assay (ELISA) was used in the measurement of the allopregnanolone level. It showed that the allopregnanolone level was increased by AC-5216 (2 and 4 nM) and the increase was reversed by PK11195 (1.5 nM). Collectedly, it firstly indicated that the effects of PK11195 on meningioma were relevant to the decrease of allopregnanolone biosynthesis, which was mediated by TSPO.
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外文
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中科院(CAS)分区：
出版当年[2015]版：
大类|4 区医学
小类|4 区肿瘤学
最新[2025]版：
大类|4 区医学
小类|4 区肿瘤学
第一作者：
第一作者机构：[1]Department of Oncology, Shunde TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Foshan, Guangdong, China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Oncology, Shunde TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Foshan, Guangdong, China.[*1]Department of Oncology, Shunde TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Foshan, Guangdong 528333, China.
推荐引用方式(GB/T 7714)：
Gao Zhuo-Wei,Huang Jing-Bin,Lin Qing,et al.The effects of PK11195 on meningioma was associated with allopregnanolone biosynthesis, which was mediated by translocator protein 18 KDa.[J].Cancer biomarkers : section A of Disease markers.2016,16(1):65-9.doi:10.3233/CBM-150541.
APA：
Gao Zhuo-Wei,Huang Jing-Bin,Lin Qing,Qin Qiang,Liang Yao-Jun...&Luo Min.(2016).The effects of PK11195 on meningioma was associated with allopregnanolone biosynthesis, which was mediated by translocator protein 18 KDa..Cancer biomarkers : section A of Disease markers,16,(1)
MLA：
Gao Zhuo-Wei,et al."The effects of PK11195 on meningioma was associated with allopregnanolone biosynthesis, which was mediated by translocator protein 18 KDa.".Cancer biomarkers : section A of Disease markers 16..1(2016):65-9
