机构：[1]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, China.[2]Jinshan District Center for Disease Control and Prevention, Shanghai 201599, China.[3]Divisions of Infectious Diseases, Cincinnati Children's Hospital Medical Center, Ohio, USA.[4]Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.[5]Department of Epidemiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, Guangdong, China.
An acute gastroenteritis (AGE) outbreak caused by a norovirus occurred at a hospital in Shanghai, China, was studied for molecular epidemiology, host susceptibility and serological roles. Rectal and environmental swabs, paired serum samples and saliva specimens were collected. Pathogens were detected by real-time polymerase chain reaction and DNA sequencing. Histo-blood group antigens (HBGA) phenotypes of saliva samples and their binding to norovirus protruding proteins were determined by enzyme-linked immunosorbent assay. The HBGA-binding interfaces and the surrounding region were analysed by the MegAlign program of DNAstar 7.1. Twenty-seven individuals in two care units were attacked with AGE at attack rates of 9.02 and 11.68%. Eighteen (78.2%) symptomatic and five (38.4%) asymptomatic individuals were GII.6/b norovirus positive. Saliva-based HBGA phenotyping showed that all symptomatic and asymptomatic cases belonged to A, B, AB or O secretors. Only four (16.7%) out of the 24 tested serum samples showed low blockade activity against HBGA-norovirus binding at the acute phase, whereas 11 (45.8%) samples at the convalescence stage showed seroconversion of such blockade. Specific blockade antibody in the population played an essential role in this norovirus epidemic. A wide HBGA-binding spectrum of GII.6 supports a need for continuous health attention and surveillance in different settings.
基金：
The study was supported by the National Natural
Science Foundation of China (grant numbers 31771007, 81773975); Natural
Science Foundation of Guangdong Province, China (grant numbers
2019A1515010951); and Science and Technology Commission of Shanghai
Municipality (grant numbers 201940428).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|4 区医学
小类|3 区公共卫生、环境卫生与职业卫生4 区传染病学
最新[2025]版：
大类|4 区医学
小类|4 区传染病学4 区公共卫生、环境卫生与职业卫生
第一作者：
第一作者机构：[1]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang X F,Chen J R,Song C L,et al.Characterization of a hospital-based gastroenteritis outbreak caused by GII.6 norovirus in Jinshan, China.[J].Epidemiology and infection.2020,148:e289.doi:10.1017/S0950268820002538.
APA：
Zhang X F,Chen J R,Song C L,Xie D J,Tan M...&Dai Y C.(2020).Characterization of a hospital-based gastroenteritis outbreak caused by GII.6 norovirus in Jinshan, China..Epidemiology and infection,148,
MLA：
Zhang X F,et al."Characterization of a hospital-based gastroenteritis outbreak caused by GII.6 norovirus in Jinshan, China.".Epidemiology and infection 148.(2020):e289
