机构：[1]Medical Center of Hip, Luoyang Orthopedic-Traumatological Hospital (Orthopedics Hospital of Henan Province), China[2]Guangzhou University of Chinese Medicine, China[3]Institute of Orthopaedics of Guangzhou University of Chinese Medicine, China[4]The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, China深圳市中医院深圳医学信息中心
Trauma-induced osteonecrosis of the femoral head (TIONFH) is characterized by femoral head collapse accompanied by degenerative changes of the hip. We previously reported that miR-93-5p expression is abnormally high in TIONFH patients, but the role of miR-93-5p in the TIONFH process remains unclear. Herein, we investigated the role of miR-93-5p in TIONFH in a rabbit model. Bone marrow mesenchymal stem cells (BMSCs) were used for both in vivo and in vitro experiments. A rabbit model of TIONFH was injected with BMSCs transfected with miR-93-5p inhibitor. In addition, both an miR-93-5p mimic and negative control were transfected into BMSCs. Expression of miR-93-5p was significantly increased in the model group compared with control samples. An miR-93-5p inhibitor induced the expression of BMP-2 and ALP. Furthermore, expression of osteogenesis-related markers (BMP-2, OPN, RUNX-2, and Osterix) was higher in the miR-93-5p inhibitor group, as revealed by qPCR and western blotting. Additionally, in vitro experimentation revealed that an miR-93-5p mimic decreased BMP-2 and OPG expression, but increased RANKL expression. In summary, the miR-93-5p inhibitor could promote osteogenic differentiation by increasing BMP-2 expression during the development of TIONFH. Thus, miR-93-5p may have potential as a therapeutic target for TIONF treatment.This article is protected by copyright. All rights reserved.
基金：
This work was supported by the National Science
Foundation of China (grant numbers 81774348 and
81874477), Science and Technology Project of Henan
Province (grant number 162102310364) and Special
Project of Traditional Chinese Medicine Research in
Henan Province (grant number 2016zy2027).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|4 区生物
小类|4 区生化与分子生物学
最新[2025]版：
大类|4 区生物学
小类|4 区生化与分子生物学
第一作者：
第一作者机构：[1]Medical Center of Hip, Luoyang Orthopedic-Traumatological Hospital (Orthopedics Hospital of Henan Province), China[2]Guangzhou University of Chinese Medicine, China
共同第一作者：
通讯作者：
通讯机构：[1]Medical Center of Hip, Luoyang Orthopedic-Traumatological Hospital (Orthopedics Hospital of Henan Province), China[*1]Medical Center of Hip, Luoyang Orthopedic-Traumatological Hospital (Orthopedics Hospital of Henan Province), No. 82 Qiming South Road, Luoyang 471002, Henan Province, China
推荐引用方式(GB/T 7714)：
Ying Zhang,Zhikun Zhuang,Qiushi Wei,et al.Inhibition of miR-93-5p promotes osteogenic differentiation in a rabbit model of trauma-induced osteonecrosis of the femoral head.[J].FEBS open bio.2021,doi:10.1002/2211-5463.13218.
APA：
Ying Zhang,Zhikun Zhuang,Qiushi Wei,Peifeng Li,Jitian Li...&Wuyin Li.(2021).Inhibition of miR-93-5p promotes osteogenic differentiation in a rabbit model of trauma-induced osteonecrosis of the femoral head..FEBS open bio,,
MLA：
Ying Zhang,et al."Inhibition of miR-93-5p promotes osteogenic differentiation in a rabbit model of trauma-induced osteonecrosis of the femoral head.".FEBS open bio .(2021)
