机构：[1]Department of Spine Surgery, Center forOrthopaedic Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, P.R. China中山大学附属第六医院[2]College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, P.R. China[3]Zhuhai PrecisionMedical Center, Zhuhai People’s Hospital (Zhuhai Hospital Affiliated with Jinan University)Jinan University, Zhuhai, Guangdong, P.R. China[4]The Biomedical Translational Research Institute, Faculty of Medical Science, Jinan University, Guangzhou, Guangdong, P.R. China[5]Vascular Department, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, P.R. China[6]Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, P.R. China[7]Guangdong Provincial Key Laboratory of Bone and JointDegeneration Disease, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong, P.R. China[8]Department of Anesthesiology, Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong, P.R. China[9]Department of Anesthesiology, Sun Yat-senMemorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, P.R. China中山大学附属第二医院
Traumatic spinal cord injury (SCI) is a devastating condition marked by permanent motor, sensory, and autonomic dysfunction, in which the inflammatory response serves an important and preventable role. High mobility group box-1 (HMGB1) is a potent regulator of inflammation in numerous acute and chronic inflammatory conditions.; however, the role of HMGB1 in SCI remains unclear. The present study aimed to characterize the temporal dynamics of HMGB1 release after SCI, to investigate the role of spinal microglia activation in mediating the effects of HMGB1 on SCI, and to explore the therapeutic potential of intrathecal anti-HMGB1 polyclonal antibody on alleviating SCI. The present study demonstrated that HMGB1 expression was increased immediately after traumatic injury of a primary spinal neuron culture. It was found that neutralizing HMGB1 significantly ameliorated SCI pathogenesis and hind limb paralysis. Moreover, the levels of a number of pro-inflammatory cytokines in the SCI lesion were reduced when local HMGB1 was blocked by anti-HMGB1 antibody. In addition, the injured neuron-derived conditioned medium increased TNF-alpha secretion and the NF-kappa B pathway in the BV2 microglia cell line via HMGB1. Collectively, these results indicated that HMGB1 served an important role in SCI inflammation and suggested the therapeutic potential of an anti-HMGB1 antibody for SCI.
基金：
National Natural Science Foundation
of China (grant nos. 81201434 and 82072513), the Department
of Science and Technology of Guangdong Province (grant no.
S2012040007947), and the Science and Technology Program of
Guangzhou (grant no. 202102080182).
第一作者机构：[1]Department of Spine Surgery, Center forOrthopaedic Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, P.R. China[*1]Department of Spine Surgery, Center forOrthopaedic Surgery, The SixthAffiliated Hospital of SunYat-senUniversity, 26 Erheng Road,Yuan Village,Guangzhou, 510655,P.R. China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Spine Surgery, Center forOrthopaedic Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, P.R. China[8]Department of Anesthesiology, Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong, P.R. China[9]Department of Anesthesiology, Sun Yat-senMemorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, P.R. China[*1]Department of Spine Surgery, Center forOrthopaedic Surgery, The SixthAffiliated Hospital of SunYat-senUniversity, 26 Erheng Road,Yuan Village,Guangzhou, 510655,P.R. China.[*2]Department of Anesthesiology, Nanfang Hospital, Southern Medical University, 1838 GuangzhouAvenue North, Guangzhou 510515,P.R. China.[*3]Department of Anesthesiology, SunYat-sen Memorial Hospital, Sun Yat-senUniversity, 107YanjiangWest Road, Guangzhou, Guangdong 510120,P.R. China.
推荐引用方式(GB/T 7714)：
Chen Ke-Bing,Chang Min-Min,Wang Sheng-Li,et al.High mobility group box-1 serves a pathogenic role in spinal cord injury via the promotion of pro-inflammatory cytokines[J].JOURNAL OF LEUKOCYTE BIOLOGY.2021,110(6):1131-1142.doi:10.1002/JLB.3MA0721-007R.
APA：
Chen, Ke-Bing,Chang, Min-Min,Wang, Sheng-Li,Li, Yong-Xin,Wang, Yi-Xi...&Ma, Wei-Ying.(2021).High mobility group box-1 serves a pathogenic role in spinal cord injury via the promotion of pro-inflammatory cytokines.JOURNAL OF LEUKOCYTE BIOLOGY,110,(6)
MLA：
Chen, Ke-Bing,et al."High mobility group box-1 serves a pathogenic role in spinal cord injury via the promotion of pro-inflammatory cytokines".JOURNAL OF LEUKOCYTE BIOLOGY 110..6(2021):1131-1142
