Exploring the molecular targets and mechanisms of [10]-Gingerol for treating triple-negative breast cancer using bioinformatics approaches, molecular docking, and in vivo experiments
机构：[1]Department of Breast Disease, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China大德路总院乳腺科大德路总院乳腺科广东省中医院[2]The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China广东省中医院[3]Department of Breast Disease, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China大德路总院乳腺科大德路总院乳腺科广东省中医院[4]Department of Clinical Laboratory, Yuebei People’s Hospital, Shaoguan, China[5]Department of Breast Disease, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China[6]Department of Pharmacy, Nanfang Hospital Baiyun Branch, Guangzhou, China
Background: Triple-negative breast cancer (TNBC) is the most aggressive among breast cancer subtypes with the worst prognosis. Ginger is widely used in pharmaceuticals and as food. Its anticancer properties are known, but the mechanism is still unclear. [10]-Gingerol is one of the main phenolic compounds isolated from ginger. Studying the biological effects of [10]-Gingerol is of great significance to understand the efficacy of ginger. Methods: In this study, the therapeutic effects of [10]-Gingerol on TNBC cells were studied using network pharmacology, molecular docking, and in vitro experiments, and the target and mechanism of action were explained. Results: A total of 48 targets of ginger for the treatment of TNBC were found. These targets might interfere with the growth of TNBC by participating in many pathways, such as endocrine resistance, progesterone-mediated oocyte maturation, estrogen signaling pathway, and cellular senescence. Prognostic analyses indicated that the JUN, FASN, ADRB2, ADRA2A, and PGR were the hub genes, while molecular docking predicted the stable binding of ADRB2 protein with drug compounds. Additionally, [10]-Gingerol could induce apoptosis by regulating the caspase activation. Conclusions: [10]-Gingerol affects the growth of TNBC through multiple action targets and participating in multiple action pathways. ADRB2 and apoptosis pathways might be important target pathways for [10]-Gingerol in the treatment of TNBC.
基金：
National Natural Science Foundation of China (No. 81974571) and China Postdoctoral Science Foundation (No. 2020M682683).
第一作者机构：[1]Department of Breast Disease, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China[2]The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China[3]Department of Breast Disease, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China[4]Department of Clinical Laboratory, Yuebei People’s Hospital, Shaoguan, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Breast Disease, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China[2]The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China[3]Department of Breast Disease, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China[4]Department of Clinical Laboratory, Yuebei People’s Hospital, Shaoguan, China[*1]Department of Breast Disease, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, No. 55, West Ring Road, Guangzhou 510282, China[*2]Department of Breast Disease, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510282, China
推荐引用方式(GB/T 7714)：
Huang Ping,Zhou Peijuan,Liang Yuqi,et al.Exploring the molecular targets and mechanisms of [10]-Gingerol for treating triple-negative breast cancer using bioinformatics approaches, molecular docking, and in vivo experiments[J].TRANSLATIONAL CANCER RESEARCH.2021,10(11):4680-4693.doi:10.21037/tcr-21-1138.
APA：
Huang, Ping,Zhou, Peijuan,Liang, Yuqi,Wu, Jiahua,Wu, Guosong...&Chen, Qianjun.(2021).Exploring the molecular targets and mechanisms of [10]-Gingerol for treating triple-negative breast cancer using bioinformatics approaches, molecular docking, and in vivo experiments.TRANSLATIONAL CANCER RESEARCH,10,(11)
MLA：
Huang, Ping,et al."Exploring the molecular targets and mechanisms of [10]-Gingerol for treating triple-negative breast cancer using bioinformatics approaches, molecular docking, and in vivo experiments".TRANSLATIONAL CANCER RESEARCH 10..11(2021):4680-4693
