个人中心| | 帮助
高级检索
当前位置： 首页 > 详情页

Transcriptome analysis of long non-coding RNAs reveals NR_015556 lncRNA is a novel regulator for adipocyte differentiation.

| 认领 | 导出 | |

文献详情

资源类型：
Pubmed体系：
作者：
Zuo Changqing[1,2] * ; Pan Yaqiong[1,3] * ; Leng Dan[1] ; Chen Xiuju[1] ; Dong Fanghongniu[1] ; Lin Zhanying[1] ; Dai Zhong[1,2,*2] ; Wang Zonggui[4,*1] ;
机构： [1]Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, And School of Pharmacy, Guangdong Medical University, Dongguan, 523808, PR China [2]Key Laboratory of Traditional Chinese Medicine and New Pharmacy Development, Guangdong Medical University, Dongguan, 523808, PR China [3]Department of Pharmacy, Dongguan Liaobu Hospital, Guangdong Medical University, Dongguan, 523400, PR China [4]Department of Biochemistry and Molecular Biology, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Dongguan, 523808, PR China
出处：
DOI： 10.1016/j.bbrc.2022.02.069
ISSN：

关键词： NR_015556 Adipocyte differentiation Wnt10b b-catenin

摘要：
Long non-coding RNAs (lncRNAs) have gained extensive attentions due to their significant roles in diverse biological process. However, the potential functions of lncRNAs participation in adipocyte differentiation have not been fully explored. In the present study, we globally profiled lncRNA expression using lncRNA microarray and identified 1745 lncRNA probes with differential expression on day 0 and day 4 post-induction in both C3H10T1/2 mesenchymal stem cells and 3T3-L1 preadipocytes. Furthermore, we showed that stable shRNA knockdown (KD) of NR_015556, a novel lncRNA that was significantly down-regulated in adipocyte differentiation, promoted adipocyte differentiation by increasing the number of lipid droplets and adipocyte markers such as Fabp4, Adipsin and Fasn. Mechanistically, NR_015556 KD attenuated the expression of Wnt signaling components Wnt10b and non-phospho (active) β-catenin, and elevated adipocyte master factors Ppar-γ and C/EBPα levels. Conversely, pharmacological activation of Wnt10b-β-catenin signaling by LiCl suppressed NR_015556 KD-induced enhancement of adipocyte differentiation and Ppar-γ and C/EBPα expression levels. Taken together, these results indicate that down-regulation of NR_015556 promotes adipocyte differentiation through inhibiting Wnt10b-β-catenin signaling pathway and then elevating Ppar-γ and C/EBPα triggered transcriptional cascades.Copyright © 2022 Elsevier Inc. All rights reserved.

基金：
This work was supported by Guangdong Provincial Natural Science Foundation, China (No. 2018A0303130203), Key Project of Guangdong Medical University (No. GDMUZ201802), Discipline Construction Project of Guangdong Medical University (No. 4SG21002G), and National Innovation Training Program for College Students, China (No. 202010571025, 202010571029).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类 | 3 区 生物学
小类 | 3 区 生物物理 4 区 生化与分子生物学
最新[2025]版：
大类 | 4 区 生物学
小类 | 4 区 生化与分子生物学 4 区 生物物理
第一作者：
第一作者机构： [1]Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, And School of Pharmacy, Guangdong Medical University, Dongguan, 523808, PR China [2]Key Laboratory of Traditional Chinese Medicine and New Pharmacy Development, Guangdong Medical University, Dongguan, 523808, PR China
共同第一作者：
通讯作者：
通讯机构： [1]Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, And School of Pharmacy, Guangdong Medical University, Dongguan, 523808, PR China [2]Key Laboratory of Traditional Chinese Medicine and New Pharmacy Development, Guangdong Medical University, Dongguan, 523808, PR China [4]Department of Biochemistry and Molecular Biology, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Dongguan, 523808, PR China [*1]Department of Biochemistry and Molecular Biology, Guangdong Medical University, No.1 Xincheng Avenue, Songshan Lake Industrial and Tech Park, Dongguan, 523808, China. [*2]Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, And School of Pharmacy, Guangdong Medical University, Dongguan, 523808, PR China.
推荐引用方式(GB/T 7714)：
Zuo Changqing,Pan Yaqiong,Leng Dan,et al.Transcriptome analysis of long non-coding RNAs reveals NR_015556 lncRNA is a novel regulator for adipocyte differentiation.[J].Biochemical and biophysical research communications.2022,601:79-85.doi:10.1016/j.bbrc.2022.02.069.
APA：
Zuo Changqing,Pan Yaqiong,Leng Dan,Chen Xiuju,Dong Fanghongniu...&Wang Zonggui.(2022).Transcriptome analysis of long non-coding RNAs reveals NR_015556 lncRNA is a novel regulator for adipocyte differentiation..Biochemical and biophysical research communications,601,
MLA：
Zuo Changqing,et al."Transcriptome analysis of long non-coding RNAs reveals NR_015556 lncRNA is a novel regulator for adipocyte differentiation.".Biochemical and biophysical research communications 601.(2022):79-85

相关文献

[1]β-Catenin Sustains and Is Required for YES-associated Protein Oncogenic Activity in Cholangiocarcinoma. [2]Quercetin induces MGMT+ glioblastoma cells apoptosis via dual inhibition of Wnt3a/β-Catenin and Akt/NF-κB signaling pathways [3]Urolithin B suppresses tumor growth in hepatocellular carcinoma through inducing the inactivation of Wnt/β-catenin signaling. [4]GLUL stabilizes N-Cadherin by antagonizing β-Catenin to inhibit the progresses of gastric cancer [5]Activation of Wnt/β-catenin pathway causes insulin resistance and increases lipogenesis in HepG2 cells via regulation of endoplasmic reticulum stress. [6]Caveolin-1 Promotes Chemoresistance of Gastric Cancer Cells to Cisplatin by Activating WNT/β-Catenin Pathway. [7]MNX1 promotes cell proliferation and activates Wnt/β-catenin signaling in colorectal cancer. [8]Therapeutic role of long non-coding RNA TCONS_00019174 in depressive disorders is dependent on Wnt/β-catenin signaling pathway. [9]Oligo-peptide I-C-F-6 inhibits hepatic stellate cell activation and ameliorates CCl4-induced liver fibrosis by suppressing NF-κB signaling and Wnt/β-catenin signaling. [10]中医临床名词术语 第2部分：外科学

资源点击量：2018 今日访问量：0 总访问量：645 更新日期：2024-07-01 建议使用谷歌、火狐浏览器 常见问题

版权所有©2020 广东省中医院 技术支持：重庆聚合科技有限公司 地址：广州市越秀区大德路111号