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FADH2-mediated radical polymerization amplification for microRNA-21 detection

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机构： [1]School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, PR China [2]Nanjing Lishui District Hospital of Traditional Chinese Medicine, Nanjing 211200, PR China [3]Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China [4]School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen, Guangdong 518060, PR China

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关键词： FADH2 Fluorometric Atom transfer radical polymerization (ATRP) Signal amplification microRNA-21

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For early diagnosis of disease, ultrasensitive mircoRNA-21 detection has considerable potential. In this paper, an ultra-sensitive fluorescence detection method for microRNA was developed by atom transfer radical polymerization (ATRP). This ATRP reaction was first initiated by using flavin mononucleotide (FADH2). The DNA probe 1 modified with amino group was fixed on the magnetic nanoparticle Fe3O4, and microRNA-21 was added to form the probe 1-microRNA-21. Another carboxy-modified DNA 2 forms a sandwich structure with the bound microRNA-21. Two terminally modified DNA types are used as microRNA probes, using complementary base pairing to form a stable super-sandwich structure between the DNA probe and the microRNA. Under optimal conditions, microRNA was detected in PBS buffer with a detection limit of 0.19 fM. And even in 10% of human serum, microRNA-21 can be detected with a detection limit of 47.8 fM. Results show that this method has high selectivity, efficiency and stability, which broad application prospect in microRNA ultra-sensitive detection.Copyright © 2023 Elsevier B.V. All rights reserved.

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出版当年[2022]版：

大类 | 2 区化学

小类 | 2 区光谱学

最新[2025]版：

大类 | 2 区化学

小类 | 2 区光谱学

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第一作者机构： [1]School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, PR China

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