机构：[1]Guangdong Key Laboratory for Genome Stability & Disease Prevention, School of Pharmaceutical Sciences, Shenzhen University Health Science Center, Shenzhen 518060, China[2]Department of Pharmacy, The Second Clinical Medical College (Shenzhen People’s Hospital), Jinan University, Shenzhen 518020, China深圳市康宁医院深圳市人民医院深圳医学信息中心[3]Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou 310008, China[4]Department of Oncology, Hubei Provincial Corps Hospital, Chinese People Armed Police Forces, Wuhan 430061, China[5]School of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Estates Building, 10 Sassoon Road, Hong Kong 00852, People’s Republic of China[6]School of Biomedical Engineering, Southern Medical University, Guangzhou 510515, China
An innovative signal amplification strategy assisted by RNase H is described here for the detection of DNA targets in a universal fashion. A tailor-made RNA molecular beacon (RMB) conjugated with a fluorescence resonance energy transfer (FRET) pair (fluorophore and quencher) was designed, characterized, and combined with the employment of RNase H. Its performance is compared to that of other nucleases including Exonuclease III and T7 exonuclease. Fluorometry, performed best at excitation/emission wavelengths of 490/520 nm, gives an amazingly low detection limit of 23 fM for target DNA. The method was verified by the determination of human hemochromatosis (HFE) gene. It is perceived that the method represents a versatile tool for the detection of a wide range of targets.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81602625, 21505039, 21705076]; Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2016A030310096]; Science and Technology Planning Project of Guangdong Province [2017A010105013]; Pearl River S&T Nova Program of Guangzhou [201710010011]; Shenzhen Science and Technology Project [JCYJ20170302145059926]
第一作者机构：[1]Guangdong Key Laboratory for Genome Stability & Disease Prevention, School of Pharmaceutical Sciences, Shenzhen University Health Science Center, Shenzhen 518060, China
通讯作者：
推荐引用方式(GB/T 7714)：
Shao-Xiang Wang,Kai-Sheng Liu,Yi-Fei Lou,et al.RNase H meets molecular beacons: an ultrasensitive fluorometric assay for nucleic acids[J].MICROCHIMICA ACTA.2018,185(8):doi:10.1007/s00604-018-2909-0.
APA：
Shao-Xiang Wang,Kai-Sheng Liu,Yi-Fei Lou,Shao-Qi Wang,Yong-Bo Peng...&Xiao Wang.(2018).RNase H meets molecular beacons: an ultrasensitive fluorometric assay for nucleic acids.MICROCHIMICA ACTA,185,(8)
MLA：
Shao-Xiang Wang,et al."RNase H meets molecular beacons: an ultrasensitive fluorometric assay for nucleic acids".MICROCHIMICA ACTA 185..8(2018)
