机构：[1]Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China[2]University of Science and Technology of China, Hefei 230026, PR China[3]Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, PR China广东省中医院
Synthetic artificial DNA probes are excellent architectural scaffolds which can be assembled into various nanostructures for further applications. A DNA walker is usually designed in response to certain external stimuli, and can significantly enhance the obtained signals. Herein, we have developed a novel DNA walker biosensor for the detection of target DNA with enzymatic recycling cleavage. By using the target DNA as a driving force, a DNA star trigon nanostructure is formed on the electrode surface, which can be recognized by a nicking endonuclease. The DNA walker is then activated to move around for the cleavage reactions on the electrode. The resulting DNA biosensor shows excellent analytical performance with a low limit of detection of 1 fM. Good selectivity and practical utility are also demonstrated. This approach has many other advantages such as ease of use, low cost, mild conditions, and may hold great potential for a range of applications in the future.
基金：
National Natural Science
Foundation of China (Grant no. 81771929), and the Scientific Research
Instrument Developing Project of the Chinese Academy of Sciences
(Grant no. YJKYYQ20170067).
第一作者机构：[1]Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China[2]University of Science and Technology of China, Hefei 230026, PR China
通讯作者：
通讯机构：[1]Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China[2]University of Science and Technology of China, Hefei 230026, PR China[*1]Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China.
推荐引用方式(GB/T 7714)：
Chai Hua,Xu Jiarui,Xu Jianhua,et al.Star trigon structure-aided DNA walker for amplified electrochemical detection of DNA[J].ELECTROCHEMISTRY COMMUNICATIONS.2019,99:51-55.doi:10.1016/j.elecom.2019.01.003.
APA：
Chai, Hua,Xu, Jiarui,Xu, Jianhua,Ding, Shaohua,Tang, Yuguo&Miao, Peng.(2019).Star trigon structure-aided DNA walker for amplified electrochemical detection of DNA.ELECTROCHEMISTRY COMMUNICATIONS,99,
MLA：
Chai, Hua,et al."Star trigon structure-aided DNA walker for amplified electrochemical detection of DNA".ELECTROCHEMISTRY COMMUNICATIONS 99.(2019):51-55
