Background: Dendrobium, with profound botanical importance, reveals a rich composition of bioactive compounds, including polysaccharides, flavonoids, alkaloids, and diverse amino acids, holding promise for skin regeneration. However, the precise mechanism remains elusive. Seeking a potent natural remedy for wound healing, exocyst vesicles were successfully isolated from Dendrobium. Aims of the Study: This investigation aimed to employ bioinformatics and in vivo experiments to elucidate target genes of Dendrobium-derived nanovesicles in skin wound healing, focusing on immune infiltration and senescence characteristics. Materials and Methods: C57 mice experienced facilitated wound healing through Dendrobium-derived nanovesicles (DDNVs). Bioinformatics analysis and GEO database mining identified crucial genes by intersecting immune -related, senescence -related, and PANoptosis-associated genes. The identified genes underwent in vivo validation. Results: DDNVs remarkably accelerated skin wound healing in C57 mice. Bioinformatics analysis revealed abnormal expression patterns of immune -related, senescence -related, and pan-apoptosis-related genes, highlighting an overexpressed IL -113 and downregulated IL -18 in the model group, Exploration of signaling pathways included IL -17, NF -kappa B, NOD -like receptor, and Toll -like receptor pathways. In vivo experiments confirmed DDNVs' efficacy in suppressing IL -113 expression, enhancing wound healing. Conclusion: Plant -derived nanovesicles (PDNV) emerged as a natural, reliable, and productive approach to wound healing. DDNVs uptake by mouse skin tissues, labeled with a fluorescent dye, led to enhanced wound healing in C57 mice. Notably, IL -113 overexpression in immune cells and genes played a key role. DDNVs intervention effectively suppressed IL -113 expression, accelerating skin wound tissue repair.