机构：[1]Department of Medicine, Zhengzhou University of Industry Technology, 16Xueyuan Road, Xinzheng 451100, Henan, China[2]Department of Vascular Surgery,Henan Provincial People’s Hospital, 7 Weiwu Road, Zhengzhou 450000,Henan, China[3]The Second Affiliated Hospital of Guangzhou Universityof Chinese Medicine, 111 Dade Road, Guangzhou 510120, China
Background Circular RNAs (circRNAs) have been shown to play a crucial role in tumorigenesis. In this study, we investigated the function of hsa_circ_0137008 and its underlying molecular mechanism in colorectal cancer (CRC). Methods Gene expression was conducted by quantitative real-time PCR or western blot. Functional experiments were performed by cell count kit-8, colony formation assay, wound healing, and transwell assays. Luciferase reporter assay and RNA pull-down assay were performed to investigate the molecular mechanism of hsa_circ_0137008 in CRC. In addition, the xenograft tumor model was applied to determine the role of hsa_circ_0137008 in vivo. Results Downregulation of hsa_circ_0137008 was observed in CRC tissues and cell lines. Functionally, overexpression of hsa_circ_0137008 inhibited the proliferation of CRC cells, as indicated by the inhibition of proliferative protein expression (Ki67 and PCNA), reduced cell viability and colony formation ability. Upregulation of hsa_circ_0137008 suppressed the migration, invasion, and epithelial to mesenchymal transition (EMT) of CRC cells. Mechanically, hsa_circ_0137008 negatively regulated the expression of microRNA-338-5p (miR-338-5p). Furthermore, hsa_circ_0137008 abated the miR-338-5p mediated promotion on CRC cell progression. Tumor suppressive function of hsa_circ_0137008 was validated in vivo. Conclusion These findings highlighted the fact that overexpression of hsa_circ_0137008 inhibited the progression of CRC via sponging miR-338-5p, suggesting that hsa_circ_0137008/miR-338-5p axis is a principal regulator of CRC tumorigenesis.
基金：
Traditional Chinese Medicine Bureau of Guangdong Province [20181103]; Specific Research Fund for TCM Science and Technology of Guangdong Provincial Hospital of Chinese Medicine [YN2016QJ18, YN10101902]
第一作者机构：[1]Department of Medicine, Zhengzhou University of Industry Technology, 16Xueyuan Road, Xinzheng 451100, Henan, China
通讯作者：
推荐引用方式(GB/T 7714)：
Yang Zhanfeng,Zhang Jingjing,Lu Danghui,et al.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge[J].CANCER CELL INTERNATIONAL.2020,20(1):doi:10.1186/s12935-020-1150-1.
APA：
Yang, Zhanfeng,Zhang, Jingjing,Lu, Danghui,Sun, Yan,Zhao, Xinyong...&Jiang, Zhi.(2020).Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge.CANCER CELL INTERNATIONAL,20,(1)
MLA：
Yang, Zhanfeng,et al."Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge".CANCER CELL INTERNATIONAL 20..1(2020)
