Haemoglobinopathies may interfere with the haemoglobin A(Ic) (HbA(Ic)) measurement, leading to incorrect diagnosis and inappropriate treatment. It is essential that HbA(Ic) assays are capable of identifying haemoglobinopathies. We report two cases of haemoglobin New York (HbNY) discovered through HbA(Ic) analysis using capillary electrophoresis (Capillarys 2 Flex Piercing [C2FP], Sebia). We used these samples to evaluate the ability of three other HbA(Ic) assays to identify this variant: ion-exchange high-performance liquid chromatography (Variant II Turbo [VII-T],Bio-Rad); boronate affinity high-performance liquid chromatography (Ultra 2, Trinity Biotech) and immunoassay (Cobas c501 Tina-quant Generation 3, Roche Diagnostics). Each method was used for HbA(Ic) assay of in samples from two cases of heterozygous haemoglobinopathy: beta(0)-thalassemia/HbNY (Case 1) and HbA/NY (Case 2). Only the C2FP system detected HbNY (an additional peak appeared between HbA(Ic) and HbA(0)). Clinical laboratories should be aware of the limitations of their HbA(Ic) assay methods especially in geographic areas, where haemoglobinopathy prevalence is high.
基金:
National Key Technologies R&D Program of ChinaNational Key Technology R&D Program [2012BAI37B01]; National Special R& D Program of Major New Drugs of China [2012ZX09303009-003]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81572088]; Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2015A030313340]; Foundation of Guangdong Provincial Hospital of Chinese Medicine [2014KT1593]
第一作者机构:[1]Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
通讯作者:
通讯机构:[1]Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China[*1]Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 111 Dade Road, Guangzhou 510120, China.
推荐引用方式(GB/T 7714):
Chao Yan,Wan Zemin,Wu Xiaobin,et al.Identification of haemoglobin New York by haemoglobin AIc measurement using the Sebia Capillarys 2 Flex Piercing system[J].ANNALS OF CLINICAL BIOCHEMISTRY.2017,54(1):178-182.doi:10.1177/0004563216671756.
APA:
Chao, Yan,Wan, Zemin,Wu, Xiaobin,Qiu, Feng,Wu, Xinzhong...&Huang, Xianzhang.(2017).Identification of haemoglobin New York by haemoglobin AIc measurement using the Sebia Capillarys 2 Flex Piercing system.ANNALS OF CLINICAL BIOCHEMISTRY,54,(1)
MLA:
Chao, Yan,et al."Identification of haemoglobin New York by haemoglobin AIc measurement using the Sebia Capillarys 2 Flex Piercing system".ANNALS OF CLINICAL BIOCHEMISTRY 54..1(2017):178-182