机构：[1]Department of Orthopedics, Luoyang Orthopedic Hospital of Henan Province, Orthopedic Hospital of Henan Province, No.100 Yongping Road,Zhengzhou City, Henan Province 450016, People’s Republic of China.[2]Department of Orthopedics, Guangzhou University of Chinese Medicine Third Affiliated Hospital, Guangzhou City, Guangdong Province 510375,People’s Republic of China.[3]Shenzhen Hospital (Futian) of Guangzhou University of Chinese Medicine, No. 6001, North Ring Road, Futian District,Shenzhen City, Guangdong Province 518048, People’s Republic of China.广州中医药大学深圳医院深圳市中医院深圳医学信息中心
Osteosarcoma (OS) is the most common type of primary bone tumor that mainly affects adolescents and young adults. The present study explored the role of lncRNA GAS8-AS1 in OS.
A total of 48 OS patients were selected from the 82 OS patients admitted by Luoyang Orthopedic Hospital of Henan Province between May 2010 and May 2013. Transient cell transfections, Transwell cell migration and invasion assay, RT-qPCR, and patient follow-up were carried out during the research.
The results showed that GAS8-AS1 was downregulated, while UCA1 was upregulate in cancer tissues in comparison to adjacent non-cancer tissues of OS patients. GAS8-AS1 was not affected by clinical stage. Follow-up study showed that downregulated GAS8-AS1 in cancer tissues was closely correlated with poor survival. GAS8-AS1 and UCA1 were inversely correlated in cancer tissues. Overexpression of UCA1 failed to affect the expression of GAS8-AS1, while overexpression of GAS8-AS1 led to downregulated expression of UCA1 in OS cells, while the molecular mediators between these two lncRNAs are unknown. Overexpression of GAS8-AS1 did not affect OS cell proliferation but significantly inhibited cancer cell migration and invasion. Overexpression of UCA1 promoted the migration and invasion of OS cells and attenuated the effects of overexpressing GAS8-AS1.
Therefore, GAS8-AS1 may inhibit OS cell migration and invasion by downregulating oncogenic UCA1.
第一作者机构：[1]Department of Orthopedics, Luoyang Orthopedic Hospital of Henan Province, Orthopedic Hospital of Henan Province, No.100 Yongping Road,Zhengzhou City, Henan Province 450016, People’s Republic of China.
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通讯作者：
推荐引用方式(GB/T 7714)：
Zha Zhuqing,Han Qingmin,Liu Wenjing,et al.lncRNA GAS8-AS1 downregulates lncRNA UCA1 to inhibit osteosarcoma cell migration and invasion.[J].JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH.2020,15(1):doi:10.1186/s13018-020-1550-x.
APA：
Zha Zhuqing,Han Qingmin,Liu Wenjing&Huo Shaochuan.(2020).lncRNA GAS8-AS1 downregulates lncRNA UCA1 to inhibit osteosarcoma cell migration and invasion..JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH,15,(1)
MLA：
Zha Zhuqing,et al."lncRNA GAS8-AS1 downregulates lncRNA UCA1 to inhibit osteosarcoma cell migration and invasion.".JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH 15..1(2020)
