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Integral Analyses of Competing Endogenous RNA Mechanisms and DNA Methylation Reveal Regulatory Mechanisms in Osteosarcoma.

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WOS体系：
Pubmed体系：

收录情况： ◇ SCIE

作者：
Wu Tingrui[1] * ; Wei Bo[1] * ; Lin Hao[1] ; Zhou Boan[1] ; Lin Tao[1] ; Liu Qianzheng[1] ; Sang Hongxun[2] ; Liu Huan[3,4] ; Huang Wenhua[1,5,6] ;
机构： [1]Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China. [2]Department of Orthopaedics, Shenzhen Hospital, Southern Medical University, Shenzhen, China. [3]Department of Orthopaedics, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, China. [4]National Traditional Chinese Medicine Clinical Research Base, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, China. [5]Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China. [6]Guangdong Medical Innovation Platform for Translation of 3D Printing Application, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
出处：
DOI： 10.3389/fcell.2021.763347
ISSN：

关键词： bioinformatics osteosarcoma competing endogenous RNA circRNA methylation

摘要：
Background: Osteosarcoma (OS) is the most common primary malignant bone tumour in children and adolescents, with rapid growth, frequent metastasis, and a poor prognosis, but its pathogenesis has not been fully elucidated. Exploring the pathogenesis of OS is of great significance for improving diagnoses and finding new therapeutic targets. Methods: Differentially expressed circRNAs (DECs), miRNAs (DEMs), methylated DNA sites (DMSs), and mRNAs (DEGs) were identified between OS and control cell lines. GSEA of DEGs and functional enrichment analysis of methylated DEGs were carried out to further identify potential biological processes. Online tools were used to predict the miRNA binding sites of DECs and the mRNA binding sites of DEMs, and then construct a circRNA-miRNA-mRNA network. Next, an analysis of the interaction between methylated DEGs was performed with a protein-protein interaction (PPI) network, and hub gene identification and survival analysis were carried out. The expression pattern of circRNA-miRNA-mRNA was validated by real-time PCR. Results: GSEA and functional enrichment analysis indicated that DEGs and methylated DEGs are involved in important biological processes in cancer. Hsa_circ_0001753/has_miR_760/CD74 network was constructed and validated in cell lines. Low expression levels of CD74 are associated with poor overall survival times and show good diagnostic ability. Conclusion: Methylated DEGs may be involved in the development of OS, and the hsa_circ_0001753/has_miR_760/CD74 network may serve as a target for the early diagnosis of and targeted therapy for OS.Copyright © 2021 Wu, Wei, Lin, Zhou, Lin, Liu, Sang, Liu and Huang.

基金：
National Natural Science Foundation of China (61427807), Guangdong Basic and Applied Research Foundation (2020B151512001), Sichuan Applied Basic Research Project (2018JY0402), Luzhou municipal people’s government-southwest medical university science and technology strategic cooperation project (2018LZXNYD-ZK19), Natural Science Foundation of Guangdong Province Science and Technology Department (2020A1515010003), Sanming Project of Medicine in Shenzhen (SZSM201612019), The Young Innovative Talents Project of Guangdong Higher Education Institutions (2021KQNCX023), “Peaking Plan” for the reconstruction of high-level hospital at Affiliated Hospital of Guangdong Medical University and Special Fund for High-level Talents (Shizhen Zhong Team) of the People’s Government of Luzhou-Southwestern Medical University.
语种：
外文
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中科院(CAS)分区：
出版当年[2020]版：
大类 | 2 区 生物
小类 | 2 区 发育生物学 3 区 细胞生物学
最新[2025]版：
大类 | 2 区 生物学
小类 | 2 区 发育生物学 3 区 细胞生物学
JCR分区：
出版当年[2019]版：
Q1 DEVELOPMENTAL BIOLOGY Q2 CELL BIOLOGY
最新[2023]版：
Q1 DEVELOPMENTAL BIOLOGY Q2 CELL BIOLOGY

影响因子： 最新[2023版] 最新五年平均 出版当年[2019版] 出版当年五年平均 出版前一年[2018版] 出版后一年[2020版]

第一作者：
第一作者机构： [1]Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
共同第一作者：
通讯作者：
通讯机构： [1]Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China. [3]Department of Orthopaedics, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, China. [4]National Traditional Chinese Medicine Clinical Research Base, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, China. [5]Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China. [6]Guangdong Medical Innovation Platform for Translation of 3D Printing Application, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
推荐引用方式(GB/T 7714)：
Wu Tingrui,Wei Bo,Lin Hao,et al.Integral Analyses of Competing Endogenous RNA Mechanisms and DNA Methylation Reveal Regulatory Mechanisms in Osteosarcoma.[J].FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY.2021,9:doi:10.3389/fcell.2021.763347.
APA：
Wu Tingrui,Wei Bo,Lin Hao,Zhou Boan,Lin Tao...&Huang Wenhua.(2021).Integral Analyses of Competing Endogenous RNA Mechanisms and DNA Methylation Reveal Regulatory Mechanisms in Osteosarcoma..FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,9,
MLA：
Wu Tingrui,et al."Integral Analyses of Competing Endogenous RNA Mechanisms and DNA Methylation Reveal Regulatory Mechanisms in Osteosarcoma.".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 9.(2021)

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